Ozonation Pre-treatment Evaluation for Xylanase Crude Extract Production from Corncob under Solid-State
| Main Authors: | Mardawati, Efri, Pratiwi, Surya Martha, Andoyo, Robi, Rialita, Tita, Djali, Mochamad, Cahyana, Yana, Sukarminah, Een, Hanidah, In-In, Setiasih, Imas Siti |
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| Format: | Article Journal |
| Bahasa: | eng |
| Terbitan: |
, 2017
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/1167599 |
Daftar Isi:
- Xylanases are a highly exploited enzyme in industries, including food and chemical industry. Xylanases can be utilized in catalyzing the endohydrolysis of 1,4-β-xylosidic linkages in xylan, lignocellulosic component to produce xylose-monomer. This research aims to optimize xylanase production from an alternative substrate, corncob. Corncob contains 41.17% of hemicellulose, the polymer of xylan. Xylanases are produced through solid state fermentation by Trichoderma viride. The ratio between substrate and moistening solution was 0.63 g/mL with fermentation temperature 32,8OC. Variables varied include incubation time and pre-treatment (using autoclave, ozonation, a combination of ozonation and autoclave, also without pre-treatment). Xylanase activity was measured by DNS method using 1% of xylan as substrate standard. The result showed that the best incubation time is 36 h with 14403.8707 U/mg protein for specific xylanase activity by using autoclave as pre-treatment. Ozonation pre-treatment process can increase the enzyme activity of xylanase.