Preliminary flagellin gene transfection into tumor cells - attempts of generating anti-tumor response in experimental model
| Main Authors: | Wędrowska, Ewelina, Wojciechowska, Marzena, Goede, Arkadiusz, Wędrowski, Mateusz, Waśniowski, Paweł, Chmielarski, Maciej, Piskorska, Elżbieta, Zukow, Walery |
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| Format: | Article |
| Terbitan: |
, 2017
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| Online Access: |
https://zenodo.org/record/1161365 |
Daftar Isi:
- Wędrowska Ewelina, Wojciechowska Marzena, Goede Arkadiusz, Wędrowski Mateusz, Waśniowski Paweł, Chmielarski Maciej, Piskorska Elżbieta, Zukow Walery. Preliminary flagellin gene transfection into tumor cells - attempts of generating anti-tumor response in experimental model. Journal of Education, Health and Sport. 2017;7(12):429-444. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1161365 http://ojs.ukw.edu.pl/index.php/johs/article/view/5225 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 1223 (26.01.2017). 1223 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Authors 2017; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 02.12.2017. Revised: 15.12.2017. Accepted: 31.12.2017. Preliminary flagellin gene transfection into tumor cells - attempts of generating anti-tumor response in experimental model Ewelina Wędrowska1, Marzena Wojciechowska1, Arkadiusz Goede1, Mateusz Wędrowski2,3, Paweł Waśniowski3, Maciej Chmielarski1, Elżbieta Piskorska4, Walery Zukow5 1Department of Gene Therapy, Faculty of Medicine, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, Poland 2Department of Positron Emission Tomography and Molecular Diagnostics, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, Poland 3Nuclear Medicine Department, Oncology Centre, Bydgoszcz, Poland 4Department of Pathobiochemistry and Clinical Chemistry, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, Poland 5Faculty of Earth Sciences, Nicolaus Copernicus University, Torun, Poland Corresponding author: Ewelina Wędrowska Zakład Genoterapii Collegium Medicum UMK 85-094 Bydgoszcz, ul. M. Skłodowskiej-Curie 9 tel. +48 517-545-893 e-mail: ewelina.wedrowska@gmail.com Abstract The development of new research techniques, especially molecular ones, creates hopes for improved treatment efficacy and a better prognosis in lung cancer. The starting point for very important experiments in the field of immunotherapy is the sensitization of the immune system to the tumor antigen. The aim of the study was to determine whether using flagellin can induce innate antitumor effective, and thus, increase the immunogenicity of the tumor. The test material was the established mouse cell line LLC, derived from Lewis lung cancer. Vectors were constructed by cloning FliC inserts into the pCDH-MSC-T2A Puro plasmid. The cells were transfected directly and indirectly (with pseudoviruses, produced by previously transfected packaging cells HEK 293T). The transfection efficiency was confirmed by RT-PCR. The cells thus prepared were implanted subcutaneously in mice. The control group received non-transfected LLC cells. Then, after 6 weeks, the mice were scarified. The animals were sectioned to isolate the tumor and lungs in which the presence or absence of metastases was assessed. In mice that received immunotherapeutic vaccines containing transfected LLC cells, less tumor mass was found or a complete lack of it, and the lifespan was noticeably prolonged. In addition, no metastases occurred in the group vaccinated with cells transfected with direct technique. It can be concluded that flagellin is effective as an adjuvant in the process of presenting tumor antigens to T cells Thus, in the light of recent studies and reports, it is likely that in this experiment effectively increased immunogenicity of the tumor. Activating anti-tumor cellular responses using flagellin is a promising target for lung cancer immunotherapy. Key words: flagellin, lung cancer, dendritic cells, transfection, anti-tumor response