PARN and TOE1 constitute a 3′ end maturation module for nuclear non-coding RNAs
| Main Authors: | Son, Ahyeon, Park, Jong-Eun, Kim, V. Narry |
|---|---|
| Format: | info dataset |
| Bahasa: | eng |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/1066619 |
Daftar Isi:
- HeLa cells were cultured in DMEM (Welgene) supplemented with 9% fetal bovine serum (Welgene). HeLa cells were transfected with 20 nM of siRNAs for four days using Lipofectamine 3000 (Thermo Fisher Scientific). Equal amounts of four different siRNAs were used for each knockdown. In the combinatorial knockdown, we mixed multiple siRNA pools to have a final concentration of 20 nM per siRNA pool. Total RNAs were extracted from siRNA-transfected HeLa cells using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol and treated with DNase I (Takara). mTAIL-seq libraries were prepared as previously described (Lim et al., 2016). Amplified cDNA libraries were sequenced on an Illumina MiSeq platform with 50% of the PhiX control library (Illumina). The uploaded file includes both intensity and sequence information for spike-ins and libraries used in the mTAIL-seq analysis. These data can be processed with Tailseeker 3.1.7 (Chang, 2017) according to the standard workflow of the software. The source codes and container images are available from Zenodo (https://zenodo.org/record/887547; doi:10.5281/zenodo.887546).