UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN SIRSAK (Annona muricata L.)TERHADAP Shigella flexneri SECARA IN VITRO
| Main Author: | ., Erika Fitriani |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Jurnal Mahasiswa PSPD FK Universitas Tanjungpura
, 2015
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| Online Access: |
http://jurnal.untan.ac.id/index.php/jfk/article/view/9040 http://jurnal.untan.ac.id/index.php/jfk/article/view/9040/8983 |
Daftar Isi:
- Diarrhea remains cause of health problem in developingcountries such as Indonesia because the mortality rate keep increasing.Diarrhea could be more serious when it becomes bloody diarrhea or alsoknown as shigellosis. Soursop (Annona muricata L.) has been used bymost of Indonesian people to treat many diseases. The society of Kutai inKalimantan choosed soursop leaves to treat diarrhea. Objective: The aimof this study was to investigate the antibacterial activity of ethanol extractsof soursop leaves against Shigella flexneri, determined the secondarymetabolite compounds, and determined the minimum inhibitoryconcentration (MIC) of ethanol extracts of soursop leaves to inhibit thegrowth of Shigella flexneri. Method: Soursop leaves was extracted bymaceration method using 70% ethanol. Chemical compounds of thisextract were determined by phytochemical screening. Antibacterial activitytest was determined by Kirby-Bauer Disc Diffusion method. This studyused various concentration consist of 500 mg/mL, 600 mg/mL, 700mg/mL, 800 mg/mL, 900 mg/mL, and 1000 mg/mL. Ciprofloxacin 5 μg/diskwas used as positive control while negative control used DMSO 10%.Result: Based on phytochemical screening, ethanol extracts of soursopleaves contained alkaloids, phenols, flavonoids, saponins, tannins, andtriterpenoids. Ethanol extracts of soursop leaves didn’t showedantibacterial activity against the growth of Shigella flexneri. Conclusion:Ethanol extracts of soursop leaves didn’t has antibacterial activity againstShigella flexneri.Keywords: Antibacterial, Ethanol extracts of soursop leaves, Shigellaflexneri1)Medical School, Faculty of Medicine, Tanjungpura University,Pontianak, West Kalimantan.2)Departement of Biology Pharmacy, Departement of Pharmacy, Facultyof Medicine, Tanjungpura University, Pontianak, West Kalimantan.3)Departement of Dermatovenerology, Medical School, Faculty ofMedicine, Tanjungpura University, Pontianak, West Kalimantan.