Evaluation of uniformity, variability, and stability of agronomic traits of doubledd haploid rice lines resulting from anther culture
| Main Author: | SASMITA, PRIATNA; Indonesian Center for Rice Research (ICRR). Jl. Raya 9 Sukamandi, Subang 41256, West Java, Indonesia. Tel. +62-260-520157; + Fax. +62-260-520158 |
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| Format: | Article info application/pdf eJournal |
| Terbitan: |
“Bioscience Communityâ€Â, School of Graduates, Sebelas Maret University, Surakarta
, 2010
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| Online Access: |
http://jurnal.pasca.uns.ac.id/index.php/nubios/article/view/51 http://jurnal.pasca.uns.ac.id/index.php/nubios/article/view/51/51 |
Daftar Isi:
- Sasmita P. 2010. Evaluation of uniformity, variability, and stability of agronomic traits of doubled haploid rice lines resultingfrom anther culture. Nusantara Bioscience 2: 67-72. The formation of doubled haploid lines in anther culture aims to accelerate theacquisition of pure lines. Selection of the desired traits can be done directly to the progeny of anther culture results at early generations.This experiment aims to determine agronomic traits , uniformity, and stability of the doubled haploid lines, and obtain the putativedoubled haploid lines as the material for further evaluation to obtain expected lines. The first experiments used completely randomizeddesign which was repeated five times. The treatments were 111 doubledd haploid lines of first generation of anther culture results(DH1). The second experiment used split plot design with the main plot treatments were doubled haploid lines resulting from antherculture and the sub plot treatment were the second generation of doubled haploid lines (DH2) until the fifth generation (DH5). Theresults show that each plant within the same line have uniform agronomic traits, while the plants between different lines have differentagronomic traits. The results of further evaluation on three out of 111 doubled haploid lines derived from the second to fifth generationsshow no difference between generations for each trait of the same lines. The results also show that the agronomic traits of the doubledhaploid line were stable from generation to generation.Key words: doubled haploid lines, uniform, stable, promising lines.