Kimpul (Xanthosoma spp.) characterization based on morphological characteristic and isozymic analysis
Daftar Isi:
- Nurmiyati, Sugiyarto, Sajidan. 2009. Kimpul (Xanthosoma spp.) characterization based on morphological characteristic andisozymic analysis. Nusantara Bioscience 1: 138-145. This research is aimed: (i) to know the variety of kimpul (Xanthosoma spp.) basedon morphological characteristics and isozymes analysis; (ii) to know the correlation between its genetic space based on morphologicalcharacteristics and its genetic resemblance based on isozymes-banding pattern. This research results were analyzed and described bydescriptive qualitative methods. Morphological observation was carried out in sub-District of Galur, Lendah and Girimulyo, KulonprogoDistrict, Yogyakarta. Morphological data of the kimpul plant was explored descriptively and then made dendogram. Data of isozymicbanding pattern were analyzed quantitatively based on the appearance of the band on the gel, and qualitatively based on the thickness ofthe band formed, and then made dendogram. The correlation, between its genetic distance based on morphological characteristics and itsgenetic resemblance based on isozymes-banding pattern, were then analyzed grounded on coefficient correlation between productmomentand goodness of it criteria based on correlation. The results pointed out that morphologically, on eight observed samples whichwere consist of four different types (species), each Xanthosoma from different locations did not indicate obvious differences. Esterasewas formed four different banding-patterns, Glutamate Oxaloacetate Transaminase indicated eight different banding-patterns, andPeroxidase indicated seven different banding-patterns. Correlation between morphological data and data from EST and GOT isozymicbanding pattern were very good (0.967918 and 0.937113), While, the correlations between morphological data and POD isozymes weregood (0.892721).