Eucheuma cottonii soaking time in a solution of atonic growth stimulants and its effects on the growth rate of thallus in vitro

Main Author: Kasnir, Muhammad
Format: Article PeerReviewed Book
Bahasa: eng
Terbitan: bioflux
Subjects:
Online Access: http://repository.umi.ac.id/329/1/Peer%20Review.pdf
http://repository.umi.ac.id/329/1/FIle%20Eucheuma.pdf
http://repository.umi.ac.id/329/2/Eucheuma%20Similarity.pdf
http://repository.umi.ac.id/329/
http://www.bioflux.com.ro/docs/2021.1078-1088.pdf
Daftar Isi:
  • Abstract. The current research addresses the effect of immersion of Eucheuma cottonii in atonic growth stimulant (ZPT) solution. The study aimed to determine the effect of the dose and duration of immersion in atonic ZPT solution on the length and weight growth rates and on the survival of E. cottonii in vitro. The research had been conducted at the Seaweed Tissue Culture Laboratory, Research Center of Brackishwater Aquaculture, Takalar for 28 days. The method used was an experiment with a factorial RAL design, combining 2 factors: (A) the immersion duration (with several levels: 0, 6, 12, 18 and 24 hours), and (B) the dose of atonic ZPT (with several levels: 5, 10 and 15 ppm). The data observed included the rate of explant weight gain, the rate of increase in explant length and the survival rate of E. cottonii explants. Data were analyzed using a one-way ANOVA and continued with the Least Significant Difference (LSD) test, in order to identify a significant difference of the means. The highest weight growth rate (RGRW) in the solution at 5 ppm was found after 12 and 18 hours of immersion. Key Words: seaweed, atonic ZPT dose, immersion, growth rate