NEWCASTLE DISEASE MATERNAL ANTIBODIES DETECTION IN BROILERS
| Main Authors: | Permana, Ida Bagus Ketut Indra, Suardana, Ida Bagus Kade, Nindhia, Tjokorda Sari |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2022
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| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/86555 https://ojs.unud.ac.id/index.php/buletinvet/article/view/86555/48421 |
Daftar Isi:
- Nevertheless, it is often found that the after vaccination antibody titers is not as expected due to the existance of maternal antibodies. A passive maternal antibodies can inhibit formation of imunoglobulin and neutralize the antigen vaccines. According to that above mentioned explanation, this research aims to detect the maternal antibodies of Newcastle Disease in broilers to carefully identify when the chicken should be vaccinated. As the main object of study, 10 unvaccinated broilers would be included in this research. These samples would be grouped by 3 different times of collecting blood, namely at the age of 7th, 14th, and 21st days. Blood sampling is subsequently carried out through branchialis vein that concludes 10 serum samples from each day. The respective samples will be serologically examined by conducting the haemaglutination (HA) and haemaglutination inhibition (HI) analysis to generate the antibody titers. All the data produced in this research will statistically processed by using descriptive statistical and regression tests on the SPSS software. The results showed a decrease in the maternal antibody titer of Newcastle Disease in broilers aged 7 days with a titer of 6.1 HI log 2, to 2.8 HI log 2 at 14 days of age, and 1.8 HI log 2 at 21 days of age. The Newcastle Disease vaccination program is most appropriate when the broiler is 10 days old because the maternal antibody titer is already at the positive threshold, namely 4 HI log 2.
- Seringkali titer antibodi yang dihasilkan pascavaksinasi tidak seperti yang diharapkan, salah satu faktor penyebabnya adalah antibodi maternal. Antibodi maternal yang diperoleh secara pasif dapat menghambat pembentukan imunoglobulin, sehingga antigen vaksin akan ternetralisir. Penelitian ini bertujuan untuk untuk mendeteksi antibodi maternal Newcastle Disease pada broiler yang berguna untuk menentukan umur ayam yang paling tepat untuk divaksinasi. Penelitian ini menggunakan 10 ekor broiler belum divaksinasi yang dibagi ke dalam 3 taraf waktu pengambilan darah yaitu pada umur 7, 14, dan 21 hari melalui vena brachialis. Jumlah serum darah yang diambil dari masing-masing umur adalah 10 sampel. Serum yang diperoleh kemudian diuji secara serologis melalui uji haemaglutination (HA) dan haemaglutination inhibition (HI) untuk mendapatkan titer antibodi. Data yang diperoleh dianalisis secara deskriptif serta dilanjutkan analisis regresi menggunakan program SPSS. Hasil penelitian menunjukkan terjadi penurunan titer antibodi maternal Newcastle Disease pada broiler umur 7 hari dengan titer 6,1 HI log 2, menjadi 2,8 HI log 2 pada umur 14 hari, dan 1,8 HI log 2 pada umur 21 hari. Program vaksinasi Newcastle Disease paling tepat dilakukan pada saat broiler berumur 10 hari karena titer antibodi maternal sudah berada pada ambang batas positif yaitu 4 HI log 2.