Histopatologi Hati Tikus yang Terpapar Logam Berat Timbal
| Main Authors: | Karina, Elisabeth, Berata, I Ketut, Setiasih, Ni Luh Eka |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2022
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| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/86320 https://ojs.unud.ac.id/index.php/buletinvet/article/view/86320/48405 |
Daftar Isi:
- Timbal merupakan salah satu bahan pencemar udara yang sangat berbahaya. Toksisitas logam berat Pb dapat menimbulkan dampak yang berat, salah satunya pada organ hati. Penelitian ini bertujuan untuk mengetahui histopatologi hati pada tikus yang terpapar logam berat timbal dengan dosis yang berbeda. Penelitian ini menggunakan tikus putih jantan dengan strain Wistar, umur 2 bulan dan berat badan 250-300 g. Tikus yang digunakan 20 ekor dibagi atas empat kelompok perlakuan yaitu kontrol (P0), Pb asetat 0,5 ppm (P1), Pb asetat 1,0 ppm (P2), dan Pb asetat 2,0 ppm (P3) selama 30 hari. Pada hari ke 31 dilakukan nekropsi lalu organ hati diambil dan dimasukkan ke dalam netral buffer formalin 10%. Setelah organ hati difiksasi, dilakukan pembuatan preparat histopatologi menggunakan pewarnaan Hematoksilin dan Eosin. Pemeriksaan histopatologi dilakukan meliputi tiga variabel lesi yaitu kongesti, degenerasi melemak, dan nekrosis. Tingkat keparahan lesi tersebut dibuat skoring yaitu 0, 1, 2 dan 3 masing-masing apabila normal, lesi ringan, sedang dan berat. Data kemudian dianalisis dengan menggunakan uji non parametik Kruskal-Wallis dan Mann-Whitney. Hasil penelitian menunjukkan bahwa pemberian logam timbal dengan kandungan 0,5 ppm, 1,0 ppm, dan 2,0 ppm menyebabkan lesi histopatologi hati tikus berupa kongesti dan nekrosis yang signifikan dibandingkan kontrol, kecuali lesi degenerasi melemak.
- Lead is one of the most dangerous air pollutants. Lead toxicity can have a severe impact, one of which is on the liver. The purpose of this research was to determine the liver histopathology of rats exposed to lead at different doses. This study used white rats of the Wistar strain, 2 months old and weighing 250-300 g. The rats used were 20 given four treatments, control (P0), 0.5 ppm Pb acetate (P1), 1.0 ppm Pb acetate (P2), and 2.0 ppm Pb acetate (P3) for 30 days. On day 31, a necropsy was performed and the liver was removed and put into 10% neutral buffered formalin. After the liver was fixated, histopathological preparations were made using Hematoxylin and Eosin. Histopathological examination staining with three variables measured, which were congestion, fatty degeneration, and necrosis. The severity of the lesions was scored as 0, 1, 2, and 3 which could cause normal, mild, moderate, and severe lesions. The data was then analyzed using the Kruskal-Wallis and Mann-Whitney non-parametric tests. The result of this research showed that the administration of lead containing 0.5 ppm, 1.0 ppm, and 2.0 ppm had an effect on the histopathology of the liver of rats lesions as significant congestion and necrosis compared to controls, except for fatty degenerating lesions.