GROSS PATHOLOGY AND HISTOPATHOLOGY DESCRIPTION OF IMMUNE ORGANS OF BALI DUCK AFTER EXPERIMENTAL INFECTION WITH AVIAN ORTHOAVULAVIRUS 1 TABANAN-1/ARP/2017 ISOLATE
| Main Authors: | Al Ma'arif, M. Farhan, Adi, Anak Agung Ayu Mirah, Winaya, Ida Bagus Oka |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2022
|
| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/86256 https://ojs.unud.ac.id/index.php/buletinvet/article/view/86256/46996 |
Daftar Isi:
- This research aims to determine the gross pathological and histopathological changes of the thymus, bursa of Fabricius, and spleen of bali ducks infected with newcastle disease virus or Avian orthoavulavirus 1 Tabanan-1/ARP/2017 isolate. This research used 15 male bali ducks aged one week. After the acclimatization period for two weeks and the measurement of ND antibody titer in duck serum showed results below 23 HI units, 10 ducks were infected with AOAV-1 Tabanan-1/ARP/2017 isolate at a dose of 0.5 ml/27 HA units intraocularly (P1) and 5 other ducks as the control (P0) were given 0.5 ml of phosphate buffered saline by the same route. Then necropsy was performed on the dead ducks and gross pathology examination was performed on its immune organs. That organs were processed and stained for Hematoxylin-Eosin, then performed histopathology examination. The results showed that infection with AOAV-1 Tabanan-1/ARP/2017 isolate in bali ducks could cause gross pathological changes in the form of necrotic foci lesions in the spleen and atrophy in the thymus. Meanwhile, the histopathological appearance showed severe depletion of lymphoid cells in the thymus and bursa of Fabricius, and mild lymphoid cell depletion in the spleen.
- Penelitian ini bertujuan untuk mengetahui perubahan patologi anatomi dan histopatologi organ timus, bursa Fabricius, dan limpa itik bali yang diinfeksi virus newcastle disease atau Avian orthoavulavirus 1 isolat Tabanan-1/ARP/2017. Penelitian ini menggunakan 15 ekor itik bali jantan berumur satu minggu. Setelah periode aklimatisasi selama dua minggu dan pengukuran titer antibodi ND dalam serum itik menunjukkan hasil di bawah angka 23 HI unit, selanjutnya sejumlah 10 ekor itik diinfeksikan dengan AOAV-1 isolat Tabanan-1/ARP/2017 dengan dosis 0,5ml/27 HA unit secara intraokular (P1) dan 5 ekor itik lainnya diberikan 0,5 ml larutan phosphate buffered saline melalui rute yang sama sebagai kontrol (P0). Nekropsi kemudian dilakukan pada itik yang mati dan dilakukan pemeriksaan patologi anatomi terhadap organ pertahanannya. Selanjutnya organ tersebut diproses dan dipulas dengan pewarnaan Hematoksilin-Eosin, lalu dilakukan pemeriksaan histopatologi. Hasil penelitian menunjukkan bahwa infeksi AOAV-1 isolat Tabanan-1/ARP/2017 pada itik bali dapat menyebabkan perubahan patologi anatomi berupa lesi foki nekrotik pada limpa dan atrofi pada timus. Sementara pada gambaran histopatologi menimbulkan deplesi sel limfoid berderajat parah pada organ timus dan bursa Fabricius, serta deplesi sel limfoid berderajat ringan pada organ limpa.