Histopatologi Limpa Ayam Petelur Pascavaksinasi Avian Influenza H5N1 Isolat dari Bali
| Main Authors: | Wardani, Putu Intan Kusuma, Kencana, Gusti Ayu Yuniati, Winaya, Ida Bagus Oka, Supartika, I Ketut Eli |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2022
|
| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/85642 https://ojs.unud.ac.id/index.php/buletinvet/article/view/85642/48406 |
Daftar Isi:
- Avian Influenza H5N1 termasuk ke dalam kelompok penyakit menular strategis dan bersifat zoonosis mematikan baik pada hewan maupun manusia yang terinfeksi. Salah satu strategi utama dalam pencegahan AI di Indonesia yaitu dengan vaksinasi. Efektivitas vaksin AI akan lebih baik apabila strain virus dalam vaksin yang digunakan homolog dengan strain virus yang ada di lapangan. Penelitian ini bertujuan untuk mengetahui histopatologi limpa ayam petelur pascavaksinasi AI-H5N1 isolat dari Bali dengan parameter diameter folikel limfoid pulpa putih limpa. Sebanyak 36 ekor ayam petelur strain Novogen Brown digunakan untuk sampel penelitian. Sampel dibagi menjadi 2 kelompok yaitu P0/kelompok kontrol tanpa perlakuan dan P1 kelompok dengan perlakuan vaksinasi AI-H5N1 isolat dari Bali. Masing-masing kelompok terdiri dari 18 ekor ayam petelur. Ayam dipelihara sejak berusia 1 hari. Vaksinasi dilakukan setelah ayam berumur 5 minggu dan di booster pada umur 10 minggu dengan dosis vaksin 0,5 ml/ekor. Pengambilan sampel organ limpa dilakukan tiga minggu pascavaksinasi booster untuk pembuatan preparat histopatologi. Data hasil pemeriksaan preparat histopatologi limpa ayam petelur dianalisis dengan uji-t. Hasil penelitian pengukuran pada organ limpa, menunjukkan rerata diameter folikel limfoid pulpa putih limpa ayam petelur dengan perlakuan vaksinasi AI-H5N1 isolat dari Bali memiliki diameter lebih besar dibandingkan dengan kelompok ayam yang tidak divaksinasi. Simpulan dari penelitian ini adalah vaksinasi AI-H5N1 isolat Bali berpengaruh nyata terhadap penambahan diameter folikel limfoid pulpa putih limpa ayam petelur pascavaksinasi.
- Avian Influenza H5N1 belongs to a group of strategic infectious diseases and is a deadly zoonosis in both infected animals and humans. One of the main strategies for preventing AI in Indonesia is vaccination. The effectiveness of the AI ??vaccine will be better if the virus strain in the vaccine used is homologous to the viral strain in the field. This study aimed to determine the histopathology of the spleen of laying hens after AI-H5N1 vaccination isolates from Bali with the parameters of the diameter of the lymphoid follicles of the white pulp of the spleen. A total of 36 laying hens of the Novogen Brown strain were used for the research sample. The sample was divided into 2 groups, namely P0/control group without treatment and P1 group with AI-H5N1 vaccination treatment, isolate from Bali. Each group consisted of 18 laying hens. Chickens are reared since the age of 1 day. Vaccination was carried out after the chickens were 5 weeks old and boosted at 10 weeks with a vaccine dose of 0.5 ml/bird. Sampling of the spleen was carried out three weeks after booster for histopathological preparations. Data from the histopathological examination of laying hens spleen were analyzed by t-test. The results of the measurement of the spleen organ showed that the average diameter of the spleen white pulp lymphoid follicles of laying hens treated with AI-H5N1 vaccination isolates from Bali had a larger diameter than the unvaccinated group of chickens. The conclusion from this study was that AI-H5N1 vaccination from Bali isolates had a significant effect on increasing the diameter of the white pulp lymphoid follicles of the spleen of laying hens after vaccination.