SEROPREVALENCE AND PROFILE ANTI-VIRUS OF NEWCASTLE DISEASE POST-VACCINATION ON LOCAL CHICKEN IN BOBONARO DISTRICT TIMOR-LESTE
| Main Authors: | Joao, Alberto Agustinho Pereira Da Costa, Astawa, I Nyoman Mantik, Adi, Anak Agun Ayu Mirah |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2022
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| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/73015 https://ojs.unud.ac.id/index.php/buletinvet/article/view/73015/44818 |
Daftar Isi:
- The objective of this study was to determine anti-Newcastle disease virus (NDV) antibody titres in sera samples of pre-vaccinated and post-vaccinated local chickens in the villages of Lahomean, Odomau Raifun and Ritabou, Bobonaro District Timor-Leste. Eighty sera samples of pre-vaccinated and 79 samples of post-vaccinated chickens were collected from village chicken farms bred extensively in all four villages. The sampling techniques adopted were simple random and purposive sampling methods to select subjects based on predetermined criteria. Antibody titers against NDV in serum samples were examined by using hemagglutination test (HA) and hemagglutination inhibition test (HI). The data obtained in this studi were presented descriptively. The results showed that of 80 sera samples collected from pre-vaccinated chickens, 53 (66.25%) sera samples were seronegative with the antibody titers of < 23 (21 – 23) HI units and 27 (33,75%) sera samples were seropositive with antibody titers of 24 – 210 HI units. As for 79 sera samples collected from post-vaccinated chickens, 46 (57%) samples were seronegaitive with the antibody titers of 21 – 23 HI and 33 (43%) sera samples were seropositive with antibody titer of 24 – 211 HI units. The results showed that presence of anti-NDV antibody in prevaccinated chickens was very likely due to the natural infection and vaccination increased the titers and the number of seropositive chickens.
- Penelitian ini bertujuan untuk mengetahui titer antibodi prevaksinasi maupun pascavaksinasi terhadap penyakit Newcastle disease (ND) pada ayam kampung di desa Lahomean, Odomau Raifun dan Ritabou Kabupaten Bobonaro Timor-Leste. Sampel serum prevaksinasi sebanyak 80 sampel dan pascavaksinasi sebanyak 79 sampel diambil dari ayam kampung yang diternakan secara ekstensif di keempat desa tersebut. Penentuan sampel penelitian dilakukan dengan teknik simple random dan purposive sampling untuk memilih subjek berdasarkan kriteria yang telah ditentukan. Titer antibodi anti-virus ND diperiksa dengan uji hemaglutinasi (HA) dan uji hambatan hemaglutinasi (HI). Data yang diperoleh dari penelitian ini disajikan secara deskriptif. Hasil penelitian menunjukkan bahwa dari 80 sampel serum prevaksinasi, 53 sampel (66,25%) menunjukkan seronegatif dengan titer antibodi < 23 (21 – 23) HI unit dan 27 sampel (33.75%) menunjukkan seropostif dengan titer antibodi 24 – 210 HI unit. Sementara itu, dari 79 sampel serum pasca-vaksinasi 46 (57%) sampel menunjukkan seronegatif dengan titer antibodi 21 – 23 HI dan 33 (43%) menunjukkan seropositif dengan titer antibodi 24 – 211 HI unit. Hasil tersebut menunjukkan bahwa sebagian kecil ayam yang belum divaksin pernah terpapar virus Newcastle disease secara alami dan vaksinasi dapat meningkatkan titer dan jumlah ayam yang seropostif terhadap virus ND.