HISTOPATHOLOGICAL CHANGES SPLEEN IN KAMPUNG CHICKEN GIVEN HERBAL MEDICINE OF ASHITABA AND AVIAN INFLUENZA VACCINE
| Main Authors: | Victoria, Velia Chyntia, Sudira, I Wayan, Winaya, Ida Bagus Oka |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2022
|
| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/69145 https://ojs.unud.ac.id/index.php/buletinvet/article/view/69145/44878 |
Daftar Isi:
- This study aims to determine the effect of giving ashitaba leaf herbal medicine as seen from the histopathological picture of native chicken spleen vaccinated against avian influenza disease vaccine. This research uses a complete random design. The treatment consisted of five treatment groups namely without ashitaba (control), with an ashitaba dose of 50; 100; 200, and 400 mg / head were dissolved with water until the volume is 100 ml and given treatment for 14 days. On the 21st day, all groups of native chickens were vaccinated against the avian influenza vaccine. On the 42nd day, native chickens were euthanasia by cervical dislocation, then the spleen was taken to see changes in the histopathological structure. Then it continued with Hematoxylin-Eosin (HE) staining and was tested under a microscope at 400x magnification. Variables that are seen from the proliferation of lymphoid follicles on white pulp by using a scaling scale for histopathological changes. The research data were analyzed by analysis non parametric Kruskal-Wallis. The results showed that there was a significant change in spleen histopathology between treatment P0 (control) and treatment given the herbal ashitaba leaf. Giving the herbal ashitaba leaf at a dose of 400 mg/head/day improves the body defense system of native chickens vaccinated with avian influenza.
- Penelitian ini bertujuan untuk mengetahui efek pemberian jamu daun ashitaba dilihat dari gambaran histopatologi limpa ayam kampung (Gallus gallus domesticus) yang divaksinasi dengan vaksin avian influenza (AI). Penelitian ini menggunakan rancangan acak lengkap. Perlakuan terdiri dari lima kelompok perlakuan yaitu tanpa ashitaba (kontrol), dengan dosis ashitaba 50; 100; 200, dan 400 mg/ekor yang dilarutkan dengan air sampai volumenya 100 ml dan diberikan perlakuan selama 14 hari. Pada hari ke-21, semua kelompok ayam kampung divaksinasi vaksin avian influenza. Hari ke-42, ayam kampung percobaan di euthanasia dengan cara dislokasi os cervicalis, kemudian limpa diambil untuk melihat perubahan struktur histopatologi. Kemudian dilanjutkan dengan pewarnaan Hematoxylin-Eosin (HE) dan diperiksa di bawah mikroskop dengan pembesaran 400x. Variabel yang diamati dilihat dari adanya proliferasi folikel limfoid pada pulpa putih dengan menggunakan skala skoring perubahan histopatologisnya. Data hasil penelitian dianalisis dengan analisis non parametrik Kruskal-Wallis. Hasil penelitian menunjukkan terdapat perubahan histopatologi limpa yang signifikan antara perlakuan P0 (kontrol) dengan perlakuan yang diberikan jamu daun ashitaba. Pemberian jamu daun ashitaba dengan dosis 400mg/ekor/hari memperbaiki sistem pertahanan tubuh ayam kampung yang divaksinasi avian influenza.