HEMATOLOGY PROFILE RATTUS NORVEGICUS THAT GIVEN INTENSIVE TRAINING AND EXTRACT OF MUSA PARADISIACA FORMATYPICA
| Main Authors: | Tangkas, Putu Jodi Wiraguna, Suarsana, Nyoman, Gunawan, I Wayan Nico Fajar |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2021
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| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/60225 https://ojs.unud.ac.id/index.php/buletinvet/article/view/60225/41087 |
Daftar Isi:
- Free radicals come from metabolic processes and external factors that can cause unstable blood cells. Antioxidants can protect blood cells from free radical attack, which comes from banana peels. The purpose of this study was to determine the effect of giving Musa paradisiaca formatypica to the blood profile of rattus norvegicus given intensive training. The study design uses a completely randomized design method (CRD) with a sample of 27 male Rattus norvegicus (200-225 grams/head) divided into 3 treatments, each consisting of 9 mice, namely the control group (P0), swimming group (P1), and swimming and banana extract (P2) groups for 28 days. Blood specimens taken through the orbital vein, then a complete hematological test performed to determine the hematology profile. The results showed that hemoglobin levels and total erythrocyte P1 were significantly lower (P<0.05) than controls (P0), whereas P2 was not significantly different from controls (P0). Total leukocyte cells P1 and P2 were not significantly different, but were significantly higher (P<0.05) than P0. Neutrophils and lymphocytes P1 and P2 were significantly higher (P<0.05) than P0, but eosinophils and monocytes did not differ from P0. HCT and MCHC levels P1 treatment were significantly lower (P<0.05) than controls, whereas P2 was not different from controls (P1). MCV levels of both P1 and P2 did not differ from controls (P0). From the results of this study it can be concluded, Musa paradisiaca formatypica can maintain levels of hemoglobin and erythrocytes but have not been able to maintain leukocyte levels and leukocyte differential values.
- Radikal bebas berasal dari proses metabolisme dan faktor eksternal yang dapat menyebabkan tidak stabilnya sel darah. Antioksidan dapat melindungi sel darah dari serangan radikal bebas, yang bersumber dari kulit pisang. Tujuan dari penelitian ini untuk mengetahui pengaruh pemberian ekstrak kulit pisang kepok terhadap profil darah tikus putih yang diberikan latihan intensif. Rancangan Penelitian menggunakan metoda rancangan acak lengkap (RAL) dengan sampel 27 ekor tikus putih jantan (200-225 gram/ekor) dibagi menjadi 3 perlakuan yang masing-masing terdiri atas 9 ekor tikus, yaitu kelompok kontrol (P0), kelompok renang (P1), dan kelompok renang dan ekstrak pisang (P2) selama 28 hari. Spesimen darah diambil melalui vena orbitalis, selanjutnya dilakukan uji hematologi lengkap untuk mengentahui profil darah. Hasil penelitian menunjukkan bahwa kadar hemoglobin dan total eritrosit P1 nyata lebih rendah (P<0.05) dari kontrol (P0), sedangkan P2 tidak berbeda nyata dengan kontrol (P0). Total leukosit P1 dan P2 tidak berbeda nyata, akan tetapi nyata lebih tinggi (P<0,05) dari P0. Netrofil dan limfosit pada P1 dan P2 nyata lebih tinggi (P<0,05) dari P0, akan tetapi eosinofil dan monosit tidak berbeda dengan P0. Kadar HCT dan MCHC perlakuan P1 nyata lebih rendah (P<0,05) dari kontrol, sedangkan P2 tidak berbeda dengan kontrol (P1). Kadar MCV baik P1 maupun P2 tidak berbeda dengan kontrol (P0). Dari hasil penelitian ini dapat disimpulkan, ekstrak pisang kepok dapat mempertahankan kadar hemoglobin dan eritrosit namun belum dapat mempertahankan kadar leukosit dan nilai deferensial leukosit.