COMPARISON OF COLIFORM AND NON-COLIFORM BACTERIA CONCENTRATION IN FECES FROM VARIOUS LIFE STAGES OF LAYING HENS
| Main Authors: | Dharmajaya, Made Tubagus, Suarjana, I Gusti Ketut, Besung, I Nengah Kerta |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2020
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| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/45261 https://ojs.unud.ac.id/index.php/buletinvet/article/view/45261/36173 |
Daftar Isi:
- This study was conducted to compare the number of coliform and non-coliform bacteria in laying feces on various age groups. This study used a Randomized Block Design (RBD) with four age treatments, namely the starter, grower, developer, and layer phases, respectively. Each treatment used six replications, with a total of 24 samples. The samples obtained were diluted with 10-5 dilutions and planted on Eosin Methylene Blue Agar (EMBA) media. After incubating for 18-24 hours at 37 ° C, the bacteria count was then calculated. Data on the number of coliform and non-coliform bacterial colonies grown on EMBA media were analyzed for variety and continued with BNT test when it was significantly different. The results showed that the number of coliform and non-coliform bacteria in laying hens in various age groups was not significantly different.
- Penelitian ini dilakukan untuk membandingkan jumlah bakteri coliform dan non-coliform pada feses ayam petelur pada berbagai kelompok umur. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan empat perlakuan umur yaitu fase starter, grower, developer, dan layer. Setiap perlakuan menggunakan masing-masing enam kali ulangan dengan total 24 sampel. Sampel yang telah diperoleh, diencerkan dengan pengenceran 10-5 dan ditanam pada media Eosin Methylene Blue Agar (EMBA). Setelah diinkubasikan selama 18-24 jam pada suhu 37oC selanjutnya dilakukan penghitungan jumlah bakteri. Data jumlah koloni bakteri coliform dan non-coliform yang tumbuh pada media EMBA dianalisis ragam dan dilanjutkan dengan uji BNT apabila berbeda nyata. Hasil penelitian menunjukkan jumlah bakteri coliform dan non-coliform pada ayam petelur pada berbagai kelompok umur tidak berbeda nyata.