Penambahan Bovine Serum Albumin pada Pengencer Beltsville Thawing Solution terhadap Motilitas dan Daya Hidup Spermatozoa Babi Landrace
| Main Authors: | Suryanatha, I Made Agus, Bebas, Wayan, Laksmi, Desak Nyoman Dewi Indira |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2019
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| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/42063 https://ojs.unud.ac.id/index.php/buletinvet/article/view/42063/31069 |
Daftar Isi:
- Penelitian ini bertujuan untuk mengetahui pengaruh penambahan berbagai konsentrasi bovine serum albumin (BSA) pada pengencer betsville thawing solution (BTS)® dalam mempertahankan daya hidup dan motilitas spermatozoa babi Landrace. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan empat perlakuan yaitu T0 (kontrol): yang diberikan BTS® tanpa BSA, T1: yang diberikan BTS®ditambahkan BSA 5 mg/ml, T2: yang diberikan BTS® ditambahkan BSA 10 mg/ml dan T3: yang diberikan BTS® ditambahkan BSA 20 mg/ml. Setiap kombinasi perlakuan,diulang sebanyak lima kali, sehingga jumlah total sampel yang digunakan sebanyak 20 sampel. Variabel yang diukur dalam penelitian ini adalah daya hidup dan motilitas semen Hasil penelitian menunjukkan bahwa penambahan BSA pada pengencer BTS® dapat meningkatkan daya hidup dan motilitas spermatozoa babi Landrace. Konsentrasi yang optimal adalah 10 mg/ml dalam menjaga kestabilan dan menjaga tekanan osmosisnya agar tetap stabil
- This study aims to determine the effect of the addition of various bovine serum albumin (BSA) concentrations on betsville thawing solution (BTS)® extender to maintaining vitality and motility of spermatozoa on Landrace pigs. This study used Completely Randomized Design (CRD) with four treatments namely T0 (control): were given BTS® without BSA, T1: were given BTS® added BSA 5 mg/ml, T2: were given BTS® added BSA 10 mg/ml and T3: were given BTS® added BSA 20 mg/ml. Each treatment combination was repeated five times, with the total sample used was 20 samples. The measured variables in this study were the vitality and motility of Landrace pig semen. The results showed that the addition of BSA to BTS® diluents could increase the vitality and motility of spermatozoa on Landrace pigs. Where the optimal concentration was 10 mg/ml in keeping stability and maintaining the osmosis pressure stable