DETECTION OF STAPHILOCOCCUS SP. FROM THE PIG RESPIRATORY TRACT
| Main Authors: | Purwanti, Makselina Ayu Dwi, Besung, I Nengah Kerta, Suarjana, I Gusti Ketut |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2018
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| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/38089 https://ojs.unud.ac.id/index.php/buletinvet/article/view/38089/25379 |
Daftar Isi:
- This research was conducted for the detection of Staphylococcus sp. in the respiratory tract of pigs. Samples differentiated by sex (male pigs and female pigs). A total of 100 nasal swab samples were collected aseptically from pigs showing symptoms of respiratory disease, from pig farms in the area of Badung and Tabanan. All samples were cultured on to Blood Agar for 18-24h at 37°C. Suspected colonies were then Gram staining, primary tests (tested for catalase and oxidase) and series of biochemical tests. Statistical analysis (Chi-square) was performed to explore the differences in positive samples numbers of Staphylococcus sp. isolated between male and female pigs. Of the 100 samples, seven positive samples of Staphylococcus sp. (six of the boars and one from the female pig). Statistically, the number of boars infected with Staphylococcus sp. higher (P<0.01) than with female pigs Staphylococcus species isolated were: Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus saprophyticus, respectively.
- Penelitian ini dilakukan untuk mendeteksi Staphylococcus sp. di saluran pernapasan babi. Sampel dibedakan berdasarkan jenis kelamin (babi jantan dan babi betina). Sebanyak 100 sampel usapan hidung dikumpulkan secara aseptik dari babi yang menunjukkan gejala penyakit respirasi, yang diambil dari peternakan babi di daerah Badung dan Tabanan. Semua sampel ditanam pada media Blood Agar selama 18-24 jam pada suhu 37°C. Koloni yang dicurigai kemudian dilakukan pewarnaan Gram, uji primer (uji katalase dan oksidase), dan serangkaian tes biokimia. Analisis statistik (Chi-square) dilakukan untuk mengetahui perbedaan jumlah sampel positif Staphylococcus sp. terisolasi antara babi jantan dan babi betina. Dari 100 sampel, tujuh sampel positif Staphylococcus sp. (enam dari babi jantan dan satu dari babi betina). Secara statistik, jumlah babi jantan yang terinfeksi Staphylococcus sp. lebih tinggi (P<0,01) dibandingkan dengan babi betina. Spesies Staphylococcus yang diisolasi adalah: Staphylococcus aureus, Staphylococcus epidermidis, dan Staphylococcus saprophyticus.