STUDY OF SUB ACUTE TOXICITY OF BALI GRAPES ETHANOL EXTRACT IN WISTAR RATS
| Main Authors: | Sudimartini, Luh Made, Nico Fajar Gunawan, I Wayan, Wirata, I Wayan, Kardena, I Made, Oka Darmayudha, Anak Agung Gde, Avianti Saritjang, Amelia |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2021
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| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/36456 https://ojs.unud.ac.id/index.php/buletinvet/article/view/36456/38536 |
Daftar Isi:
- Bali grapes are a source of antioxidants with high flavonoids and anthocyanins, which have many health benefits such as lowering cholesterol and anti-cancer, inhibiting aging, and protecting the heart. Many people consume Balinese grapes by eating directly, drink in the form of juice or extract form because of its benefits. The purpose of this study was to determine the sub-acute toxicity of the ethanol extract of bali grapes (Vitis vinera cv. Alphonso lavelle) to changes in SGOT and SGPT of male Wistar rat liver. This test includes a sub-acute toxicity test and a pharmacological effect test. This research is an experimental study with a completely randomized design using a post-test-only control group design. The number of white rats used in this study were 24 rats, which were divided into four groups of control groups, groups of mice given ethanol extract of bali grapes 0.3 g / ml/kg BW (P1), groups of mice given ethanol extract of grapes bali 3 g / ml/kg BW (P2), and the group of rats that were given 30 g / ml kg BW (P3) of ethanol extract of bali grapes. Each group consisted of 6 white male rats. In this study, the grape ethanol extract was presented orally once per day for 28 days. After 28 days of treatment, Wistar rats were euthanized, and blood samples were taken through Chantus Medial Orbitalis and then put into non-EDTA tubes. The blood sample that has been centrifuged and becomes serum is then inserted into the Eppendorf tube, which is used to test the levels of SGOT and SGPT. The results of the One-Way ANOVA analysis on SGPT levels in the liver of rats showed a significant difference (P <0.05), so that further analysis was necessary using the Duncan-test. Based on the results of the analysis using the Duncan Test, changes occurred in the P3 treatment group of rats given the ethanol extract of Bali grapes at a dose of 30 g / ml/kg BW, which was significantly different from the control treatment group (P0) so that the mixture of these materials was included in the class of materials that were practically non-toxic.
- Anggur bali merupakan sumber antioksidan dengan kandungan flavonoid dan antosianin yang cukup tinggi yang memiliki banyak manfaat di bidang kesehatan seperti menurunkan kolesterol, anti kanker, menghambat penuaan dan melindungi jantung. Karena manfaatnya tersebut, buah anggur bali banyak di konsumsi masyarakat dengan cara dimakan langsung, diminum dalam bentuk jus ataupun dalam bentuk ekstrak. Tujuan dari penelitian ini adalah untuk mengetahui toksisitas sub akut ekstrak etanol buah anggur bali (Vitis vinera cv. Alphonso lavelle) terhadap perubahan SGOT dan SGPT hati tikus wistar jantan. Uji ini meliputi uji toksisitas sub-akut dan uji efek farmakologis. Penelitian ini adalah penelitian eksperimental dengan Rancangan Acak Lengkap (Completely Randomized Design) menggunakan post-test only control group design. Total tikus putih yang digunakan dalam penelitian ini adalah 24 ekor, yang dibagi menjadi empat kelompok yaitu kelompok kontrol, kelompok tikus yang diberi ekstrak etanol buah anggur bali 0,3 g/ml/kg BB (P1), kelompok tikus yang diberi ekstrak etanol buah anggur bali 3 g/ml/kgBB (P2), dan kelompok tikus yang diberi ekstrak etanol buah anggur bali 30 g/ml kg BB (P3).Masing-masing kelompok terdiri atas 6 ekor tikus putih jantan. Pada penelitian ini, pemberian ekstrak etanol buah anggur dilakukan per oral sebanyak satu kali per-hari selama 28 hari. Setelah 28 hari perlakuan, tikus wistar dieuthanasi dan sampel darah diambil melalui Chantus Medial Orbitalis kemudian dimasukkan ke dalam tabung non-EDTA. Sampel darah yang telah disentrifugasi dan menjadi serum kemudian dimasukkan kedalam tabung eppendorf digunakan untuk uji kadar SGOT dan SGPT. Hasil analisa dengan One Way ANOVA pada kadar SGPT hati tikus menunjukkan adanya perubahan yang berbeda nyata (P<0,05) sehingga perlu dilakukan analisis lanjutan dengan menggunakan Duncan-test. Berdasarkan hasil analisis menggunakan Duncan Test, perubahan terjadi pada kelompok tikus perlakuan P3 yang diberikan ekstrak etanol buah anggur bali dengan dosis 30 g/ml/kgBB berbeda nyata terhadap kelompok perlakuan kontrol (P0) sehingga campuran bahan tersebut termasuk dalam golongan bahan practically non toxic.