PHYSICOCHEMICAL CHARACTERISTICS AND ANTIMICROBIAL ACTIVITY TEST OF BACTERIOCINS ORGINATED FROM LACTIC ACID BACTERIA ISOLATES 17B ISOLATED FROM BALI CATTLE COLON
| Main Authors: | Putri, Nuria Fitrianti, Suardana, I Wayan, Utama, Iwan Harjono |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2018
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| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/35063 https://ojs.unud.ac.id/index.php/buletinvet/article/view/35063/25378 |
Daftar Isi:
- Lactic acid bacteria (LAB) is a microorganism that is not toxic and able to produce antimicrobial compounds, namely bacteriocins. This study aims to determine the physicochemical characterization of bacteriocins origin originated from lactic acid bacteria isolates 17B isolated from colon Bali cattle. The research was started by re-cultivation of isolates 17B and continued with production and precipitation of bacteriocins. Further tests were chemical and physical test that, followed by antimicrobial activity test of bacteriocin isolates 17B against Bacillus cereus FTCC 005. Data From this study are presented in tables or figure and analyzed descriptively. Results of study showed that LAB isolates 17B is a Gram-positive bacteria and catalase negative. The test also showed the bacteriocins produced isolates 17B as a protein compound indicated by the ninhydrin test positive, Molisch test negative and Lowry test positive with protein concentration was 0,11 μg/ml. Physical characterization tested using SDS PAGE showed that the test is negative. Antimicrobial activity test of bacteriocin isolates 17B showed that bacteriocin 17B has effectiveness against Bacillus cereus FTCC 005 as 18,92%.
- Bakteri asam laktat (BAL) merupakan suatu mikroorganisme yang bersifat tidak toksik dan mampu menghasilkan senyawa antimikroba, yakni bakteriosin. Penelitian dimulai dari peremajaan isolat 17B dan dilanjutkan dengan produksi dan presipitasi bakteriosin yang dilanjutkan dengan uji sifat kimiawi dan sifat fisik dari bakteriosin. Aktivitas antimikroba bakteriosin isolat 17B dilakukan terhadap bakteri indikator Bacillus cereus FTCC 005. Data hasil penelitian ini disajikan dalam bentuk tabel atau gambar dan analisis secara deskriptif. Hasil penelitian meneguhkan bahwa BAL isolat 17 merupakan bakteri Gram positif dan katalase negatif. Hasil uji sifat kimiawi bakteriosin dari isolat 17B, menunjukkan bahwa bakteriosin yang dihasilkan sebagai senyawa protein yang dibuktikan dengan uji ninhidrin positif, uji Molisch negatif, dan uji lowry positif dengan konsentrasi protein 0, 11 μg/ml. Hasil karakterisasi sifat fisik dengan menggunakan SDS PAGE menunjukkan bahwa hasil uji negatif. Uji aktivitas antimikroba terhadap bakteriosin menunjukkan efektivitas hambatan sebesar 18,92%.