ETHANOL EXTRACT OF MYRMECODIA PENDANS CAUSED HISTOLOGICAL STRUCTURE DAMAGE OF MICE KIDNEY
| Main Authors: | Manullang, Dini Hilary, Sudira, I Wayan, Berata, I Ketut, Merdana, I Made |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2018
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| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/34305 https://ojs.unud.ac.id/index.php/buletinvet/article/view/34305/25376 |
Daftar Isi:
- Chemical analysis of the ant-plant (Myrmecodia pendans) showed that plants have various chemical compounds of flavonoids, tannins, tocopherols, multimineral and polysaccharides. The aim of this study was to find out the effect of ethanolic extract of ant-plant on histopathological changes in male mice (Mus musculus) kidney. Twenty four clinically healthy mice at aged 10-12 with weight 25 to 35 g were obtained for this study. The sample were divided into four groups randomly, each treatment group consisted of six mice. Group P0 is the negative control group were given standard feed and drink, P1 is a group were given standard feed and ethanol extracts ant plant with a dose of 100 mg/kg body weight, P2 is a group were given standard feed and ethanol extracts ant plant with a dose of 200 mg/kg of body weight, P3 is a group were given standard feed and ethanol extracts ant plant with a dose of 300 mg/kg. After completion of the treatment on the day, the kidney was taken for histological preparations were made and stained with the hematoxylin-eosin method. The variables examined were haemorrhage, degeneration and necrosis in the renal proximal tubules. The Kruskal-Wallis test results showed that ethanol extract of ant-plant had a significant effect (P?0.05) on the incidence of hemorrhage in the renal tubules. Based on these results it can be concluded that the ethanol extract of ant-plant with a dose of 300 mg/kg body weight can cause kidney histopathological changes such as hemorrhage, degeneration, and necrosis.
- Analisis kimia menunjukkan bahwa tumbuhan sarang semut (Myrmecodia pendans) memiliki berbagai kandungan senyawa kimia dari golongan flavonoid, tannin, tokoferol, multimineral dan polisakarida. Penelitian ini bertujuan mengetahui pengaruh ekstrak etanol sarang semut terhadap gambaran histopatologi ginjal mencit (Mus musculus) jantan. Penelitian ini menggunakan 24 ekor mencit jantan dengan umur 10-12 minggu yang secara klinis dinyatakan sehat dengan berat 25-35 g. Secara acak seluruh mencit dibagi menjadi 4 kelompok perlakuan, masing-masing kelompok perlakuan terdiri atas 6 ekor mencit. Kelompok P0 adalah kelompok kontrol negatif yang diberikan pakan standar dan aquades, P1 adalah kelompok yang diberikan pakan standar dan ekstrak etanol sarang semut dengan dosis 100 mg/kg BB, P2 adalah kelompok yang diberikan pakan standar dan ekstrak etanol sarang semut dengan dosis 200 mg/kg BB, P3 adalah kelompok yang diberikan pakan standar dan ekstrak etanol sarang semut dengan dosis 300 mg/kg BB. Setelah perlakuan selesai pada hari ke-21, organ ginjal diambil untuk dibuat preparat histologi dan diwarnai dengan metode haematoksilin-eosin. Variabel yang diperiksa adalah perdarahan, degenerasi melemak, dan nekrosis di tubulus proksimal ginjal. Hasil uji Kruskall-Wallis menunjukkan pemberian ekstrak etanol sarang semut berpengaruh nyata (P?0,05) terhadap timbulnya perdarahan pada tubulus ginjal. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa ekstrak etanol sarang semut dengan dosis 300 mg/kg BB dapat menyebabkan perubahan histopatologi ginjal berupa perdarahan, degenerasi melemak, dan nekrosis.