IMMUNE RESPONSE OF BALI DUCK POST VACCINATION AGAINST NEWCASTLE DISEASE
| Main Authors: | Novitasari, Anindya, Suardana, Ida Bagus Kade, Sampurna, I Putu |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2018
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| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/33343 https://ojs.unud.ac.id/index.php/buletinvet/article/view/33343/25374 |
Daftar Isi:
- The purpose of this research was to know immune response of local bali duck post vaccination against Newcastle Disease, as well as the time required to form protective antibody titer. The research used completely randomized design with time as a treatment. The object of research was the serum of local bali duck. Blood was taken when pre-vaccination was done at aged three weeks old of the local Bali ducks, and then at the aged of four weeks old, vaccination was done subcutaneously. Post vaccination blood collection was performed four times in every oneweek intervals. The blood was taken from the cranial tibial vein by using 1 cc syringe. The serum was transferred to the Eppendorf tube. Then the antibody against Newcastle Disease titers were tested by using Hemaglutination inhibition (HI) test and expressed in Geometric Mean Titer (GMT) with units of Log 2. Antibody titer examination pre-vaccination was 0.83±0.408 Log 2. The mean of antibody titer at the first, second, third, and fourth week were 2±0.632 Log 2, 5.5±1.225 Log 2, 1.2±0.983 Log 2, dan 0.16±0.408 Log 2 respectively. The highest titer as well as was protective of the were reached in the second week post vaccination.
- Penelitian ini bertujuan untuk mengetahui respon imun itik terhadap vaksinasi Newcastle Disease (ND), serta waktu yang diperlukan agar terbentuk titer antibodi yang protektif. Penelitian menggunakan rancangan acak lengkap (RAL) dengan waktu sebagai perlakuan. Objek penelitian menggunakan serum itik Bali. Pengambilan darah pravaksinasi dilakukan pada umur tiga minggu, pada umur empat minggu dilakukan vaksinasi secara sub kutan. Pengambilan darah pascavaksinasi dilakukan empat kali dengan interval waktu satu minggu, diambil dari vena tibialis cranial dengan spuite satu cc, dan serum dipindahkan ke dalam tabung Eppendorf. Titer antibodi diuji dengan uji Hemaglutination inhibition (HI), dan dinyatakan dalam Geometric Mean Titer (GMT) dengan satuan Log 2. Rerata titer antibodi pravaksinasi adalah 0,83±0,408 Log 2. Sedangkan rerata titer antibodi minggu ke-1,2,3 dan 4 pasca vaksinasi adalah 2±0,632 Log 2, 5,5±1,225 Log 2, 1,2±0,983 Log 2, dan 0,16±0,408 Log 2. Dapat disimpulkan titer antibodi protektif (?2?) pada minggu ke-2 pascavaksinasi.