PRIMARY IMUNE RESPON OF LAYER POST VACCINATED WITH THE EGG DROPS SYNDOME VACCINE

Main Authors: Kencana, Gusti Ayu Yuniati, Suartha, I Nyoman, Wibawa, I Putu Wira Adi
Format: Article info application/pdf eJournal
Bahasa: eng
Terbitan: The Faculty of Veterinary Medicine, Udayana University , 2017
Online Access: https://ojs.unud.ac.id/index.php/buletinvet/article/view/31518
https://ojs.unud.ac.id/index.php/buletinvet/article/view/31518/20124
Daftar Isi:
  • This study was conducted to determine the primary immune response post vaccination using EDS inactivated vaccine polyvalent. The sample used was a commercial layer farm in the village of Tiga, regency of Bangli, Bali. A total of 25 layer which were14 weeks old vaccinated using EDS-76 inactivated vaccine containing polyvalent Newcastle disease antigen virus, infectious bronchitis and egg drop syndrome by intramuscularly injection. Examination of EDS antibody titer using serologic test by Hemagglutination Inhibition (HI) test.  Egg drops syndrome antibody titer checked four times, once before vaccination, and every week for three weeks post-vaccination to see the immune responses. The average antibody titer then analyzed using an univariate of variance test followed by a test of Least Significant Difference, Duncan test and regression analysis. The result showed an increase antibody of EDS was significantly every week post vaccination. The average antibody titers of EDS are 22,6 HI unit at one weeks post vaccination, about 25,04 HI unit at two weeks post vaccination and  26,4 HI unit at three weeks post vaccination.
  • Penelitian ini dilakukan untuk mengetahui respon imun primer ayam petelur pasca vaksinasi Egg drop syndrome.  Sampel penelitian adalah ayam petelur jenis Isa Brown yang dipelihara di Desa Tiga, Kabupaten Bangli, Provinsi Bali. Sebanyak 25 ekor ayam petelur umur 14 minggu divaksin dengan vaksin EDS inaktif polivalen (mengandung antigen virus Newcastle disease, infectious bronchitis dan egg drop syndrome). Vaksinasi dilakukan secara intramuskuler pada otot dada. Pemeriksaan titer antibodi EDS dengan uji serologi Hambatan Hemaglutinasi (HI). Titer antibodi egg drop syndrome diperiksa sebanyak  empat kali yaitu satu kali sebelum vaksinasi dan tiga kali pasca vaksinasi. Rataan titer antibodi setiap minggu dianalisis menggunakan sidik ragam dilanjutkan dengan uji beda nyata terkecil, dan analisis regresi. Hasil penelitian menunjukkan bahwa terjadi peningkatan titer antibodi yang signifikan setiap minggunya pada ayam petelur pasca divaksinasi EDS. Titer antibodi satu minggu pasca vaksinasi sebesar 22,6 HI unit, pada dua minggu pasca vaksinasi sebesar 25,04 HI unit, dan  tiga minggu  pasca vaksinasi sebesar 26,4 HI unit.