LACTIC ACID BACTERIA ISOLATE 18A PHENOTYPIC IDENTIFICATION
| Main Authors: | Suardana, I Wayan, Sukoco, Hendro, Antara, Nyoman Semadi |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2018
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| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/31406 https://ojs.unud.ac.id/index.php/buletinvet/article/view/31406/22997 |
Daftar Isi:
- Lactic Acid Bacteria (LAB) is a bacterium that has an ability to inhibit a pathogenic bacteria growth, including isolates 18A. The aim of this study is to determine the species of LAB isolates 18A by conventional tests and API 50 CH test. This study was a descriptive observational study, with lactic acid bacteria isolates 18A were obtained from bali cattle’s colon as an object. LAB isolates 18A were re-cultured on MRS media, catalase test, and Gram staining. Identification test were performed by CO2 production test, growth in the presence of 15% NaCl, growth at 10°C, and growth at pH 9.6. Confirmation test were performed using API 50 CH (Standard Analytical Profile Index). The results indicated that LAB isolate 18A were able to grow well on MRS broth media, catalase-negative, Gram-positive, and spherical-chain in shape. Identification test revealed that the bacteria is homofermentative, were able to grow at 10°C, and do not grow at pH 9.6. Fermentation test using API 50 CH showed that LAB isolates 18A were able to degrade 15 sugar components, consist of no: 5, 10, 11, 12, 13, 22, 24, 25, 26, 27, 28, 29, 30 , 31, and 39. Based on conventional identification results, it can be concluded that BAL isolates 18A is a Lactococcus. In addition, the result of API 50 CHL test showed that BAL isolates 18A is a Lactococcus lactis ssp lactis 1.
- Bakteri Asam Laktat (BAL) memiliki kemampuan dalam menghambat pertumbuhan bakteri patogen, salah satunya adalah BAL isolat 18A. Penelitian ini bertujuan untuk menentukan spesies BAL dari isolat 18A dengan uji konvensional dan uji KIT API 50 CH. Penelitian ini merupakan penelitian observasional deskriptif, dengan objek penelitian berupa bakteri asam laktat isolat 18A asal kolon sapi bali. BAL isolat 18A direkulturisasi, dengan penumbuhan pada media MRS, uji katalase, dan pewarnaan Gram. Uji identifikasi isolat dengan uji produksi gas CO2, uji pertumbuhan pada NaCL 15%, uji pertumbuhan pada Suhu 100C, dan uji pertumbuhan pada pH 9,6. Konfirmasi isolat juga dilakukan dengan uji kit API (Standard Analytical Profile Index) 50 CH. Hasil rekulturisasi menunjukkan isolate 18A mampu tumbuh dengan baik pada media MRS broth, bersifat katalase negatif, Gram positif, berbentuk bulat, dan berantai. Hasil identifikasi mengindikasikan bahwa isolat 18A bersifat homofermentatif, mampu tumbuh pada suhu 100C, dan tidak tumbuh pada pH 9,6. Uji fermentasi dengan menggunakan perangkat kit API 50 CH, menunjukkan bahwa isolat 18A mampu mendegradasi 15 komponen gula, yaitu komponen gula no : 5, 10, 11, 12, 13, 22, 24, 25, 26, 27, 28, 29, 30, 31, dan 39. Hasil identifikasi secara konvensional dapat disimpulkan bahwa isolate 18A merupakan genus Lactococcus. Dan berdasarkan uji konfirmasi menggunakan Kit API 50 CHL disimpulkan bahwa isolat 18A merupakan bakteri Lactococcus Lactis ssp lactis 1.