CONSUMPTION OF BEEF BALI CATTLE AND IT’S EFFECTS ON RATS PLASMA LIPOPROTEIN PROFILE
| Main Author: | Suarsana, I Nyoman |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
The Faculty of Veterinary Medicine, Udayana University
, 2016
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| Subjects: | |
| Online Access: |
https://ojs.unud.ac.id/index.php/buletinvet/article/view/19677 https://ojs.unud.ac.id/index.php/buletinvet/article/view/19677/13068 |
Daftar Isi:
- Consumption of beef in Indonesia is continuously increasing. Aside from being a source of protein, beef also contains all essential amino acids, vitamins, fats and cholesterol making it an ideal choice for consumers. This study aims to analyze the consumption of beef bali on rats plasma lipoprotein levels. A total of 15 male rats Spraque Dawlly average body weight of 90-100g was used in this study. They were sub-divided into five groups: a control group, without treatment (I), fed- beef group was treated from 7th day (II), fed-beef group was treated from 5th day (III), fed-beef group was treated from 3th day (IV), and fed-beef group was treated from 1st day (V). At the end of the experiment, i.e 9th days all groups of rats were euthanasia with cethamine-HCl. Blood was taken through the heart and placed in tubes containing EDTA to obtain plasma. Levels of cholesterol, triglycerides, HDL was analyzed by spectrophotometric method using cholesterol KIT (Ref10028), TGA (Ref10720P), and HDL (Ref10018). LDL levels were calculated using the formula: LDL= total cholesterol-(TG/5)-HDL. The results showed that rats given beef bali cattle for 8 days was lead to increased plasma triglyceride levels significantly (P<0.05), while cholesterol, HDL (high density lipoprotein) and LDL (low density lipoprotein) plasma levels is not increased.
- Konsumsi daging sapi di Indonesia terus mengalami peningkatan. Selain sebagai sumber protein, daging sapi juga mengandung semua asam amino esensial, vitamin, kandungan lemak dan kolesterol sehingga menjadi pilihan yang ideal bagi konsumen. Penelitian ini bertujuan untuk menganalisis konsumsi daging sapi bali terhadap kadar lipoprotein plasma tikus. Sebanyak 15 ekor tikus percobaan dibagi menjadi lima kelompok perlakuan masing-masing terdiri atas tiga ekor, yaitu kelompok kontrol tanpa diberi daging sapi (I), kelompok tikus yang diberi daging sapi mulai hari ke tujuh (II), kelompok tikus yang diberi daging sapi mulai hari ke lima (III), kelompok tikus yang diberi daging sapi mulai hari ke tiga (IV), kelompok tikus yang diberi daging sapi mulai hari ke pertama (V). Pada akhir percobaan, yaitu hari ke sembilan semua kelompok tikus dieutanasia menggunakan ketamin-HCl. Darah diambil melalui jantung dan ditempatkan pada tabung yang sudah berisi Ethylenediaminetetraacetic acid (EDTA) untuk mendapatkan Plasma. Kadar Kolesterol, Trigliserida, High-density lipoprotein (HDL) di analisis menggunakan metode spektropotometri menggunakan KIT cholesterol (Ref10028), TGA (Ref10720P), dan HDL (Ref10018) Human. Kadar Low density lipoprotein (LDL) dihitung menggunakan rumus: kolesterol total -(TG/5)-HDL. Hasil penelitian menunjukkan bahwa tikus percobaan yang diberi daging sapi bali selama delapan hari menyebabkan kadar trigliserida plasma meningkat secara nyata sedangkan kadar kolesterol, kadar HDL dan LDL plasma tidak mengalami peningkatan.