Selenoprotein W1 (SEPW1) Gene Polymorphism and Association with Mineral Content in Sheep
| Main Authors: | Astira, Adinda Wanda, Harahap, Ratna Sholatia, Sumantri, Cece, Gunawan, Asep |
|---|---|
| Other Authors: | RISTEKDIKTI |
| Format: | Article info eJournal |
| Bahasa: | eng |
| Terbitan: |
Universitas Halu Oleo
, 2023
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| Online Access: |
http://ojs.uho.ac.id/index.php/peternakan-tropis/article/view/27066 |
Daftar Isi:
- Selenoprotein W1 (SEPW1) is a protein containing selenium that has an important role in muscle growth and development through protecting myoblasts from oxidative stress. This study was aim to identify diversity of the SEPW1 gene and its association with mineral content in sheep. The samples used were 100 sheep consisting 10 samples of compass agrinak sheep (CAS), 15 samples of javanese fat tailed sheep (JFT), 30 samples of javanese thin tailed sheep (JTT), 25 samples of garut composite sheep (GCS), and 20 samples of jonggol sheep (JS). Selected mineral contents were determined using the IK method LP-04.10-LT-1.0. Identification of SEPW1 gene polymorphism will be conducted using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The size of the amplification product was 455 bp. Chi-square analys is showed that the population was in Hardy-Weinberg equilibrium. Polymorphism of SEPW1 gene were found in each breed of sheep. The SEPW1 gene’s SNP g.53617063 A>G was significantly (p<0.005) linked to selenium mineral concentration. The SEPW1 gene was potential to be used as a selection marker for mineral content in lamb.