IDENTIFICATION OF MARINE MICROALGAE FROM TAMBRAUW, WEST PAPUA
| Main Authors: | Purbani, Debora Christin, Ambarwati, Wiwik, Kusuma, Aradea Bujana, Herliany, Nurlaila Ervina |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University
, 2019
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| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/jurnalikt/article/view/25862 http://journal.ipb.ac.id/index.php/jurnalikt/article/view/25862/18991 |
Daftar Isi:
- Tambrauw merupakan salah satu Kabupaten di Papua Barat, dikenal sebagai daerah mega-biodiversitas, termasuk mikroalga laut yang memiliki peran penting dalam sistem rantai makanan di perairan. Namun di sisi lain keanekaragaman mikroalga laut di daerah ini masih belum banyak diteliti. Penelitian ini dilakukan untuk mengisolasi dan mengidentifikasi mikroalga laut dari Tambrauw berdasarkan pendekatan morfologi dan molekular. Mikroalga laut diisolasi dari sumber alami di Tambrauw, dimurnikan dan dikultur di bawah kondisi standar. Empat belas kultur mikroalga laut dipilih berdasarkan pertumbuhan, keragaman morfologi dan homogenitas. Karakteristik morfologi diamati dalam kondisi kultur menggunakan mikroskop cahaya dan hubungan filogenetik dari masing-masing strain didefinisikan sesuai dengan analisis sekuen 18S rRNA pada kelompok mikroalga eukariot dan 16S rRNA pada kelompok mikroalga prokariot. Sekuen yang dihasilkan dibandingkan dengan database yang tersedia di situs web NCBI melalui alat bioinformatika BLAST. Hasil penelitian menunjukkan similaritas yang tinggi dengan identitas urutan nukleotida yang dikenal, sebagai Monoraphidium neglectum (99%), Chlorella sorokiniana (99%), Oocystis heteromucosa (99%), Ettlia texensis (99%), Dilabifilum arthopyreniae (98%), Auxenochlorella protothecoides (99%), Trichosarcina polymorpha (98%), Scenedesmus vacuolatus (99%), Chlorella kessleri (99%), Coelastrella oocystiformis (99%), dan Foliisarcina bertiogensis (99%). Studi ini menjadi informasi dasar dalam mengungkap pola keanekaragaman mikroalga, yang sangat penting untuk mendapatkan sumber daya baru genetik untuk kepentingan industri serta studi taksonomi.
- Tambrauw as one of the regencies in West Papua, is well known as a megabiodiversity area, including marine microalgae that have an important role in the food chain system in the water. On the other hand the diversity of marine microalgae in this area has not been studied much. This study was conducted to identify marine microalgae from Tambrauw based on morphology and molecular approach. Marine microalgae were isolated from natural sources in Tambrauw, purified and cultivated under standard conditions. Fourteen cultures of marine microalgae were selected based on growth, diversity of morphology and homogeneity. The morphological characteristics were observed using light microscopy and the phylogenetic relationships of each strain were defined according to the 18S rRNA sequence analysis in the eukaryotic group and 16S rRNA in the prokaryotic microalgae group. The resultant sequences were compared with those available on the NCBI website database through the BLAST bioinformatic tool. The results showed high similarity with known nucleotide sequence identified as Monoraphidium neglectum (99%), Chlorella sorokiniana (99%), Oocystis heteromucosa (99%), Ettlia texensis (99%), Dilabifilum arthopyreniae (98%), Auxenochlorella protothecoides (99%), Trichosarcina polymorpha (98%), Scenedesmus vacuolatus (99%), Chlorella kessleri (99%), Coelastrella oocystiformis (99%), dan Foliisarcina bertiogensis (99%). This study is the basic information in revealing the diversity patterns of microalgae, which are very important to obtain new genetic resources for industrial purposes as well as for taxonomic studies.