In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant
| Main Authors: | Herlina, Tati, Supratman, Unang, Subarnas, Anas, Sutardjo, Supriyatna, Amien, Suseno, Hayashi, Hideo |
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| Format: | Article info application/pdf Document |
| Bahasa: | eng |
| Terbitan: |
Indonesian Society for Cancer Chemoprevention
, 2011
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| Online Access: |
https://ijcc.chemoprev.org/index.php/ijcc/article/view/44 https://ijcc.chemoprev.org/index.php/ijcc/article/view/44/45 |
Daftar Isi:
- Erythrina plants, locally known as “dadap ayam”, are higher plant species and have been used as a folk medicine for treatment of cancer. To prove the effectiveness of the leaves and stem bark of E. variegata as an anti-cancer agent, the assay in this research was focused on in vitro test towards breast cancer cell T47D. In the course of our continuing search for novel anti-cancer agent from Erythrina plants, the methanol extract of the leaves and stem bark of E. variegata showed significant anti-cancer activity against breast cancer cell T47D in vitro using the Sulphorhodamine B (SRB) assay. By using the anti-cancer activity to follow the separations, the methanol extract was separated by combination of column chromatography. The chemical structure of an anti-cancer compounds were determined on the basis of spectroscopic evidence and comparison with the previously reported and identified as an erythrina alkaloid (1) and isoflavonoid (2). Compounds (1-2) showed anti-cancer activity against breast cancer cell T47D used with IC50 of 1.0 and 3.3 μg/mL, respectively. This results strongly suggested that E. variegata is promising sources for anti-cancer agents. Keywords: Anti-cancer, Erythrina variegata, Leguminoseae