Antioxidant Properties and Cytotoxic Activity of Ethyl Acetate Fraction of Plectranthus amboinicus (Lour.) Spreng. Leaves on HeLa and T47D Cell Lines
| Main Authors: | Hasibuan, Poppy Anjelisa Zaitun, Sitorus, Panal, Satria, Denny, Sibuea, Rizka Damela |
|---|---|
| Other Authors: | Poppy Anjelisa Zaitun Hasibuan, Universitas Sumatera Utara |
| Format: | Article info application/pdf Document |
| Bahasa: | eng |
| Terbitan: |
Indonesian Society for Cancer Chemoprevention
, 2019
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| Online Access: |
https://ijcc.chemoprev.org/index.php/ijcc/article/view/258 https://ijcc.chemoprev.org/index.php/ijcc/article/view/258/181 |
Daftar Isi:
- Research into plants with anticancer effects is actively encouraged in orderto discover new drugs with lessertoxicity but more potent effects. The aims of study are to evaluate the antioxidant properties and to investigate the cytotoxic activity of Plectranthus amboinicus (Lour.) Spreng. leaves ethyl acetate fractions on HeLa,T47D and MCF7 cell lines. The extract was prepared by graded maceration using n-hexane and ethyl acetate. The ethyl acetate extract was fractionated in vacuum liquid chromatography with n-hexane: ethyl acetate; and ethyl acetate: methanol as mobile phase. Then, the fractions were analyzed with thin layer chromatography (TLC). The free radical scavenging activity was measured by DPPH method, the total flavonoid content was calculated by quercetin equivalent and the absorbance is measured by using UV-Visible spectrophotometry. The cytotoxic activity were determined using MTT assay. The fractions contained 5 sub fractions with same TLC profile. The fractions showed antioxidant activity by DPPH method with different IC50 values, namely: 130 μg/mL(I), 127 μg/mL(II), 137 μg/mL(III), 129 μg/mL(IV), and 124 μg/ mL(V), respectively. The measurement of total flavonoid content showed 118 mg QE/g (I), 50 mg QE/g (II), 207 mg QE/g (III), 56 mg QE/g (IV), and 55 mg QE/g (V). The IC50 of each sub fractions on HeLa cell were 77 μg/mL, 46 μg/mL, 93 μg/mL, 71 μg/mL and 476 μg/mL; for T47D cell were 1621 μg/mL, 111 μg/mL, 128 μg/mL, 150 μg/mL and 209 μg/mL; and for MCF7 were 259 μg/mL, 343 μg/mL, 575 μg/mL, 408 μg/mL and 250 μg/mL. Based on the results, the fractions derived from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. leaves exhibit antioxidant. The Fraction II from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. was the most cytotoxic on HeLa, T47D and MCF7 cell lines. It is potential to undergo further isolation of its cytotoxic compounds.Keywords : antioxidant, cytotoxic, Plectranthus amboinicul (Lour.) Spreng., ethyl acetate fractions