THE EFFECT OF ROSELLA (HIBISCUS SABDARIFFA L) TREATMENT ON IL-10 AND IL-14 SECRETION ON DIMETHYLBENZ (A) ANTHRACENE (DMBA) INDUCED RAT
| Main Author: | Nurkhasanah, Nurkhasanah |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Innovare Academic Sciences Pvt Ltd, India
, 2015
|
| Subjects: | |
| Online Access: |
http://eprints.uad.ac.id/7641/1/2..pdf http://eprints.uad.ac.id/7641/ |
Daftar Isi:
- Objective: The objective of this study was to determine the effect of ethanolic extract rosella calyx pretreatment on increasing secretion of IL-10 and IL-14 on SD rats treated with DMBA. Methods: Rats were treated with a dose of ethanol extract of roselle with 10, 50 and 100 mg / kg BW once daily for 21 days, then treated with DMBA 15mg / rat single dose. One week after DMBA treatment lymphatic organs of mice were taken and the expression of IL-10 and IL-14 were observed by immunohistochemistry using the appropriate antibodies. Further more it was observed using a microscope at 400x magnification. Result: The results showed that the obtained were analyzed using t-test to determine to what the effect of increased secretion of IL-10 and IL-14 between the control and treatment groups. Conclusion: Rosella calyx extract is potential to develop as immunomodulatory agent Keywords: Hibiscus sabdariffa, IL-10, IL14, Immunomodulator.