Kinetics Evaluation on Oleic Acid Ethyl Ester Synthesis Using Lipase From Rice Bran (Oryza sativa) and Germinated Jatropha Seeds (Jatropha curcas. L)

Main Authors: Prastowo, Indro, Hidayat, Chusnul, Hastuti, Pudji
Format: Article PeerReviewed Book
Bahasa: eng
Terbitan: Ahmad Dahlan University , 2014
Subjects:
Online Access: http://eprints.uad.ac.id/2775/1/Proceeding%20ICGWBT.pdf
http://eprints.uad.ac.id/2775/
Daftar Isi:
  • Recently, lipase has attracted some interests because of its usage in catalyzing esterification reaction for the production of biodiesel (Fatty Acid Ethyl Ester). In this research, lipase from rice bran (Oryza sativa) and germinated jatropha seeds (Jatropha curcas. L) were observed as industry needs low-priced sources of lipase. Kinetics evaluation is very important to understand and further predict the synthesis rate of Oleic Acid Ethyl Ester (OAEE) at certain period. The objective of this study was to evaluate the kinetics of OAEE synthesis catalyzed by lipase from rice bran (Oryza sativa) and germinated jatropha seeds (Jatropha curcas. L). The maximum synthesis rate (Vmax) for lipase from rice bran (Oryza sativa) and germinated jatropha seeds (Jatropha curcas. L) were 60.98 μmol/min and 49.5 μmol/min, respectively. Meanwhile, the Michaelis-Menten constant (Km) for lipase from rice bran and germinated jatropha seeds were 0.073 M and 0.124 M, respectively. Keywords : OAEE; kinetics evaluation; lipase; rice bran (Oryza sativa); germinated jatropha seeds (Jatropha curcas. L).