Similarity Check In vitro activity of immunomodulator of N-Hexane fraction of bangle (Zingiber cassumunar Roxb.) Ethanol extract
| Main Authors: | Mahfudh, Nurkhasanah, Sulistyani, Nanik, Noorlina, Noorlina |
|---|---|
| Format: | Lainnya NonPeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
World Research Journal Indore, India
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| Subjects: | |
| Online Access: |
http://eprints.uad.ac.id/20563/1/In%20vitro%20activity%20of%20immunomodulator%20of%20N-Hexane.pdf http://eprints.uad.ac.id/20563/ |
Daftar Isi:
- Zingiber cassumunar is potentially an immunomodulator. The aim of study is to determine the immunomodulatory activity of n-hexane fraction of Zingiber cassumunar ethanol extracts with a measurement of phagocytic activity and lymphocyte proliferation in vitro. Macrophage cells are isolated from peritoneum of mice. Cells were planted and added to latex and n-hexane fraction of Zingiber cassumunar ethanol extract and then painted using 20% giemsa. Lymphocyte cells are isolated from lymph. Cells were planted and given n-hexane fraction of Zingiber cassumunar ethanol extract. Incubate for 3 days using 5% CO2 incubator at 37°C. Data were analysed using SPSS with a confidence level of 95%. The results of the average percentage of phagocytic activity and phagocytic index of n-hexane fraction of Zingiber cassumunar ethanol extract of 0.025 μg/ml, 0.050 μg/ml, 0.100 μg/ml and controls were respectively 5.247% and 5.311%, 7.475% and 7.720%, 10.593% and 11.393% and 15.989% and 19.317%. The results of the average lymphocyte proliferation absorbance of 0.025 μg/ml, 0.050 μg/ml, 0.100 μg/ml and controls respectively are 0.107, 0.127, 0.126 and 0.149. The n-hexane fraction of Zingiber cassumunar ethanol extract can reduce the phagocytic activity of macrophages (p<0.05) and lymphocyte proliferation (p<0.05) in mice in vitro.