Similarity Check: Validation Of Analytical Method For Determining Ciprofloxacin’s Level In Blood Using High Performace Liquid Chromatography (HPLC)
| Main Authors: | Rahmatullah, Satrio Wibowo, Sandi, Dita Ayulia Dwi, Perwitasari, Dyah Aryani, Darmawan, Endang |
|---|---|
| Format: | Lainnya NonPeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
RJPBCS RESEARCH JOURNAL PHARMACEUTICAL, BIOLOGICAL & CHEMICAL SCIENCES
|
| Subjects: | |
| Online Access: |
http://eprints.uad.ac.id/18720/1/Validation_Of_Analytical_Method_For_Determining_Ci.pdf http://eprints.uad.ac.id/18720/ |
Daftar Isi:
- Ciprofloxacin is a broad-spectrum antibiotic, often used empirically for infection. Ciprofloxacin is an antibiotic whose activity depends on levels in blood (concentration depends), potential effect of ciprofloxacin will be optimal when ciprofloxacin’s levels in blood is high compared with its MIC. To measure the level of ciprofloxacin in the patient's blood required a simple and accurate method of analysis. This study aims to validation of analysis method for determining level of ciprofloxacin. This study use of mobile phase 2% acetic acid and acetonitrile (84: 16). HPLC conditions include HPLC devices, YMC C18 column (4,6 x 150 mm: 5 μm), UV detector 280 nm and flow rate is 1,0 mL / min. Validation parameter in this study are selectivity, accuracy, linearity, LOD and LOQ. The results of analysis method for measure levels of ciprofloxacin is selective (α > 1,9177) at time 3,845 minute. The HPLC method of ciprofloxacin meets the acceptability criteria of %recovery value is 96,9%. Eligible precision test % RSD is <3,5%. The value of r 2 on the linearity is 0.9989. LOD value is 1,1 μg/mL and LOQ value is 3,6 μg/mL. Keywords: ciprofloxacin, HPLC, validation