Peer Review ANTIANGIOGENIC EFFECTS OF WATER FRACTION OF GREEN ALGAE (Spirogyra sp.) ETHANOL EXTRACT WITH CHORIO ALLANTOIC MEMBRANE METHOD
| Main Authors: | Suryaningrum, Ketty, Widyaningsih, Wahyu |
|---|---|
| Format: | Lainnya NonPeerReviewed Image |
| Bahasa: | eng |
| Terbitan: |
Proceeding of International Conference on Drug Development of Natural Resources
|
| Subjects: | |
| Online Access: |
http://eprints.uad.ac.id/13629/1/Antiangiogenic%20Effect%20of%20Water%20Fraction....jpg http://eprints.uad.ac.id/13629/ |
Daftar Isi:
- Background. Angiogenesis plays an important role in tumor progression. Therapeutic angiogenesis is a major focus in cancer treatment. Modern treatment of cancer currently has alternative to inhibit cancer through antiangiogenesis process, it is expected to inhibit the formation of new blood vessels around the tumor, the supply of nutrients and oxygen by the blood to the tumor can be inhibited. This study aims to determine the fraction of water antiangiogenesis effect of ethanol extract of green algae (Spirogyra sp.) by using the method of chorio allantoic membrane (CAM). Methods. In this study subject were devided into 7 groups; group I of the control disc, group II on the disc+PBS control group, group III of the control disc+PBS +bFGF, variation in the concentration of the water fraction of ethanol extract of green algae (Spirogyra sp.) is group IV, V, VI, VII which is each of these group has variation of doses 100, 200, 400 and 800 μg/ml. CAM is obtained from embryonated chicken eggs aged 8-9 days, then it is given treatment bFGF (angiogenesis inductor) and water fractions of ethanol extract of green algae (Spirogyra sp.), and then incubated for 3 days. Results and Discussion. Observations are made in the macroscopic to see the inhibitory activity of blood vessels from the water fraction of ethanol extract of green algae (Spirogyra sp.). Having obtained the result of macroscopic observation, the data of the number of blood vessels were tested statistically by the method of Mann Whitney and Kruskal Wallis with 95% confidence level. Conclusions. The results showed that the water fraction of ethanol extract of green algae with doses 100, 200, 400 and 800 μg/ml can inhibit angiogenesis of embryonated chicken eggs compared to bFGF control. In conclusion, the water fraction of ethanol extract of green algae has antiangiogenesis activity in chorio allantoic membrane