Metagenomic Sequencing Analysis and Microbial Identification on Various Landcover

Main Authors: Priyadarshini, Rossyda, Syafriani, Elly, Somala, Medina Uli Alba, Hamzah, Amir
Format: Proceeding PeerReviewed Book
Bahasa: eng
Terbitan: , 2019
Subjects:
Online Access: http://repository.upnjatim.ac.id/197/1/Metgenomic_ISRM.pdf
http://repository.upnjatim.ac.id/197/7/metagenomics.pdf
http://repository.upnjatim.ac.id/197/12/Metagenomic%20Sequencing.pdf
http://repository.upnjatim.ac.id/197/
ctrlnum 197
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>http://repository.upnjatim.ac.id/197/</relation><title>Metagenomic Sequencing Analysis and Microbial Identification on Various&#xD; Landcover</title><creator>Priyadarshini, Rossyda</creator><creator>Syafriani, Elly</creator><creator>Somala, Medina Uli Alba</creator><creator>Hamzah, Amir</creator><subject>S Agriculture (General)</subject><description>Soil degradation will affect the availability of soil nutrients. Microbes role was important in supplying&#xD; soil nutrient. How is microbes supply the nutrients can be learned through the metagenome technique.&#xD; Metagenome is one of the molecular techniques that can identify microbial communities from soil&#xD; samples that are used quickly and precisely. Through the metagenome technique, not only information&#xD; about the identity of microbial species can be obtained, but also the enzymes produced, which will&#xD; affect the soil nutrients availability. This study aims to detect and identify the impact of land cover&#xD; types on microbial types. Soil samples were taken under agricultural land (LP) and agroforestry (AF).&#xD; Each type of land cover was taken at 4 (four) different locations. The method used in this research is a&#xD; molecular-based species identification method through metagenome analysis. This study showed that&#xD; there are variations in microbial species in various types of land cover. This information is important&#xD; for sustainable agricultural management in the future. Our study showed that on all of the land cover&#xD; types, Proteobacteria were the most abundant phylum in all of the land cover patterns. Among them,&#xD; s_Sphingomonas_melonis; f_Sphingomonadaceae; g_Sphingomonas; c_Alphaproteobacteria;&#xD; p_Proteobacteria; and o_Sphingomonadales were the most abundant in the LP groups, while&#xD; p_Acidobacteria and c_Deltaproteobacteria were the most abundant in AF groups. This composition&#xD; of soil metagenomic was associated with the soil physic-chemical properties, especially pH and soil&#xD; organic matter.&#xD; Keywords: metagenomic technique, soil bacteria, 16S rRNA gene amplicon, land cover types</description><date>2019</date><type>Journal:Proceeding</type><type>PeerReview:PeerReviewed</type><type>Book:Book</type><language>eng</language><identifier>http://repository.upnjatim.ac.id/197/1/Metgenomic_ISRM.pdf</identifier><type>Book:Book</type><language>eng</language><identifier>http://repository.upnjatim.ac.id/197/7/metagenomics.pdf</identifier><type>Book:Book</type><language>eng</language><identifier>http://repository.upnjatim.ac.id/197/12/Metagenomic%20Sequencing.pdf</identifier><identifier> Priyadarshini, Rossyda and Syafriani, Elly and Somala, Medina Uli Alba and Hamzah, Amir (2019) Metagenomic Sequencing Analysis and Microbial Identification on Various Landcover. In: 4th Internasional Research Month. </identifier><recordID>197</recordID></dc>
language eng
format Journal:Proceeding
Journal
PeerReview:PeerReviewed
PeerReview
Book:Book
Book
author Priyadarshini, Rossyda
Syafriani, Elly
Somala, Medina Uli Alba
Hamzah, Amir
title Metagenomic Sequencing Analysis and Microbial Identification on Various Landcover
publishDate 2019
topic S Agriculture (General)
url http://repository.upnjatim.ac.id/197/1/Metgenomic_ISRM.pdf
http://repository.upnjatim.ac.id/197/7/metagenomics.pdf
http://repository.upnjatim.ac.id/197/12/Metagenomic%20Sequencing.pdf
http://repository.upnjatim.ac.id/197/
contents Soil degradation will affect the availability of soil nutrients. Microbes role was important in supplying soil nutrient. How is microbes supply the nutrients can be learned through the metagenome technique. Metagenome is one of the molecular techniques that can identify microbial communities from soil samples that are used quickly and precisely. Through the metagenome technique, not only information about the identity of microbial species can be obtained, but also the enzymes produced, which will affect the soil nutrients availability. This study aims to detect and identify the impact of land cover types on microbial types. Soil samples were taken under agricultural land (LP) and agroforestry (AF). Each type of land cover was taken at 4 (four) different locations. The method used in this research is a molecular-based species identification method through metagenome analysis. This study showed that there are variations in microbial species in various types of land cover. This information is important for sustainable agricultural management in the future. Our study showed that on all of the land cover types, Proteobacteria were the most abundant phylum in all of the land cover patterns. Among them, s_Sphingomonas_melonis; f_Sphingomonadaceae; g_Sphingomonas; c_Alphaproteobacteria; p_Proteobacteria; and o_Sphingomonadales were the most abundant in the LP groups, while p_Acidobacteria and c_Deltaproteobacteria were the most abundant in AF groups. This composition of soil metagenomic was associated with the soil physic-chemical properties, especially pH and soil organic matter. Keywords: metagenomic technique, soil bacteria, 16S rRNA gene amplicon, land cover types
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