Metagenomic Sequencing Analysis and Microbial Identification on Various Landcover
Main Authors: | Priyadarshini, Rossyda, Syafriani, Elly, Somala, Medina Uli Alba, Hamzah, Amir |
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Format: | Proceeding PeerReviewed Book |
Bahasa: | eng |
Terbitan: |
, 2019
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Subjects: | |
Online Access: |
http://repository.upnjatim.ac.id/197/1/Metgenomic_ISRM.pdf http://repository.upnjatim.ac.id/197/7/metagenomics.pdf http://repository.upnjatim.ac.id/197/12/Metagenomic%20Sequencing.pdf http://repository.upnjatim.ac.id/197/ |
ctrlnum |
197 |
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fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>http://repository.upnjatim.ac.id/197/</relation><title>Metagenomic Sequencing Analysis and Microbial Identification on Various
Landcover</title><creator>Priyadarshini, Rossyda</creator><creator>Syafriani, Elly</creator><creator>Somala, Medina Uli Alba</creator><creator>Hamzah, Amir</creator><subject>S Agriculture (General)</subject><description>Soil degradation will affect the availability of soil nutrients. Microbes role was important in supplying
soil nutrient. How is microbes supply the nutrients can be learned through the metagenome technique.
Metagenome is one of the molecular techniques that can identify microbial communities from soil
samples that are used quickly and precisely. Through the metagenome technique, not only information
about the identity of microbial species can be obtained, but also the enzymes produced, which will
affect the soil nutrients availability. This study aims to detect and identify the impact of land cover
types on microbial types. Soil samples were taken under agricultural land (LP) and agroforestry (AF).
Each type of land cover was taken at 4 (four) different locations. The method used in this research is a
molecular-based species identification method through metagenome analysis. This study showed that
there are variations in microbial species in various types of land cover. This information is important
for sustainable agricultural management in the future. Our study showed that on all of the land cover
types, Proteobacteria were the most abundant phylum in all of the land cover patterns. Among them,
s_Sphingomonas_melonis; f_Sphingomonadaceae; g_Sphingomonas; c_Alphaproteobacteria;
p_Proteobacteria; and o_Sphingomonadales were the most abundant in the LP groups, while
p_Acidobacteria and c_Deltaproteobacteria were the most abundant in AF groups. This composition
of soil metagenomic was associated with the soil physic-chemical properties, especially pH and soil
organic matter.
Keywords: metagenomic technique, soil bacteria, 16S rRNA gene amplicon, land cover types</description><date>2019</date><type>Journal:Proceeding</type><type>PeerReview:PeerReviewed</type><type>Book:Book</type><language>eng</language><identifier>http://repository.upnjatim.ac.id/197/1/Metgenomic_ISRM.pdf</identifier><type>Book:Book</type><language>eng</language><identifier>http://repository.upnjatim.ac.id/197/7/metagenomics.pdf</identifier><type>Book:Book</type><language>eng</language><identifier>http://repository.upnjatim.ac.id/197/12/Metagenomic%20Sequencing.pdf</identifier><identifier> Priyadarshini, Rossyda and Syafriani, Elly and Somala, Medina Uli Alba and Hamzah, Amir (2019) Metagenomic Sequencing Analysis and Microbial Identification on Various Landcover. In: 4th Internasional Research Month. </identifier><recordID>197</recordID></dc>
|
language |
eng |
format |
Journal:Proceeding Journal PeerReview:PeerReviewed PeerReview Book:Book Book |
author |
Priyadarshini, Rossyda Syafriani, Elly Somala, Medina Uli Alba Hamzah, Amir |
title |
Metagenomic Sequencing Analysis and Microbial Identification on Various
Landcover |
publishDate |
2019 |
topic |
S Agriculture (General) |
url |
http://repository.upnjatim.ac.id/197/1/Metgenomic_ISRM.pdf http://repository.upnjatim.ac.id/197/7/metagenomics.pdf http://repository.upnjatim.ac.id/197/12/Metagenomic%20Sequencing.pdf http://repository.upnjatim.ac.id/197/ |
contents |
Soil degradation will affect the availability of soil nutrients. Microbes role was important in supplying
soil nutrient. How is microbes supply the nutrients can be learned through the metagenome technique.
Metagenome is one of the molecular techniques that can identify microbial communities from soil
samples that are used quickly and precisely. Through the metagenome technique, not only information
about the identity of microbial species can be obtained, but also the enzymes produced, which will
affect the soil nutrients availability. This study aims to detect and identify the impact of land cover
types on microbial types. Soil samples were taken under agricultural land (LP) and agroforestry (AF).
Each type of land cover was taken at 4 (four) different locations. The method used in this research is a
molecular-based species identification method through metagenome analysis. This study showed that
there are variations in microbial species in various types of land cover. This information is important
for sustainable agricultural management in the future. Our study showed that on all of the land cover
types, Proteobacteria were the most abundant phylum in all of the land cover patterns. Among them,
s_Sphingomonas_melonis; f_Sphingomonadaceae; g_Sphingomonas; c_Alphaproteobacteria;
p_Proteobacteria; and o_Sphingomonadales were the most abundant in the LP groups, while
p_Acidobacteria and c_Deltaproteobacteria were the most abundant in AF groups. This composition
of soil metagenomic was associated with the soil physic-chemical properties, especially pH and soil
organic matter.
Keywords: metagenomic technique, soil bacteria, 16S rRNA gene amplicon, land cover types |
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Higher Education, Universities/Pendidikan Tinggi, Unversitas Engineering/Ilmu Teknik Agriculture/Industri Pertanian Business/Bisnis |
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KOTA SURABAYA |
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JAWA TIMUR |
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