Ekspresi Rekombinan Gen Protein Selubung Pepper vein yellows virus
| Main Authors: | Apindiati, Rita Kurnia, Suastika, Gede, Mutaqin, Kikin Hamzah |
|---|---|
| Format: | Article info application/pdf Journal |
| Bahasa: | eng |
| Terbitan: |
The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)
, 2015
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| Subjects: | |
| Online Access: |
https://journal.ipb.ac.id/index.php/jfiti/article/view/10101 https://journal.ipb.ac.id/index.php/jfiti/article/view/10101/7905 |
Daftar Isi:
- Pepper vein yellows virus (PeVYV) isolate from Bali have been identified from pepper plants with chlorosis symptoms. Specific antiserum of PeVYV had not available yet commercially. One of the advance techniques in providing a source of abundant antigen for antiserum production is through molecular approach by overexpressed the coat protein gene in suitable bacterial expression system. PeVYV coat protein gene of ~650 bp in size was amplified using specific primers, then was cloned into pQE30 expression vector and was over expressed in E. coli strain M15 [pREP4]. SDS-PAGE analysis showed that the recombinant coat protein gene of PeVYV was successfully expressed protein band with size of ~25 kDa at 6 hours after induction by 0.5 mM IPTG on 37 °C.