Biological Evaluations of protoporphyrin IX, pheophorbide α, and its 1-hydroxyethyl derivates for application in photodynamic theraphy
| Main Authors: | Asmiyenti, Djaliasrin Djalil, Nunuk, Aries Nurulita, Leenawaty, Limantara, Slamet, Ibrahim, Daryono, Hadi Tjahjono |
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| Format: | Article PeerReviewed |
| Terbitan: |
, 2012
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| Subjects: | |
| Online Access: |
http://eprints.upj.ac.id/id/eprint/92/ http://www.ijppsjournal.com/Vol4Issue3/4301.pdf |
Daftar Isi:
- Protoporphyrin IX (1), pheophorbide a (3), and its 1‐hydroxyethyl derivativess (2,4) were studied in vitro as photosensitizer candidates for photodynamic therapy. Protoporphyrin IX has been indicated to have a cytotoxic effect in the absence of light excitation. The dark toxicity of 1¬4 was evaluated against normal cells (Vero), human epithelial cervix carcinomas (HeLa) and human breast cancer (T47D) cell lines, while the phototoxicity of 1¬4 was evaluated against HeLa and T47D cell lines. Moreover, the MTT assay was employed to evaluate cell viability. The 1‐hydroxyethyl derivativess showed a lower dark toxicity in the three types of cells compared to the parent molecules. It was also observed that the parent molecules were more phototoxic than those of its 1‐hydroxyethyl derivativess.