Analysis of Pigment Composition of Brown Seaweeds Collected from Panjang Island, Central Java, Indonesia

Main Authors: Heriyanto, Heriyanto, Ayu, Dita Juliadiningtyas, Yuzo, Shioi, Leenawaty, Limantara, Tatas, H P Brotosudarmo
Format: Article PeerReviewed Book
Bahasa: eng
Terbitan: , 2017
Subjects:
Online Access: http://eprints.upj.ac.id/id/eprint/105/1/b8c068c23f084f816e5caf4f34b1485a4529.pdf
http://eprints.upj.ac.id/id/eprint/105/
Daftar Isi:
  • Composition of pigments from four species of brown seaweeds (Phaeophyceae) collected from Panjang Island, Central Java, Indonesia, was investigated with spectroscopic method and reverse-phase high-performance liquid chromatography (RP-HPLC). Identification of pigments was based on their spectral and chromatographic properties and also confirmed by electrospray ionization-mass spectrometry (ESI-MS/MS) analysis. The experimental results showed that concentrations of chlorophyll a (Chl a) and total carotenoids (Cars) from brown seaweeds, estimated by spectroscopic method, varied depending on species from 1.73 mg • g–1 to 8.84 mg • g–1 and from 0.55 mg • g–1 to 4.06 mg • g–1 dry weight (dw), respectively. In addition, the order of concentrations of Chl a and total Cars in four species of seaweed was as follows: Dictyota dentata > Padina australis > Sargassum crassifolium > Turbinaria conoides. This order was in agreement with the concentrations of dominant pigments calculated by HPLC method, i.e., fucoxanthin (Fuco) (0.43 mg • g–1 to 4.11 mg • g–1 dw), Chl a (1.70 mg • g–1 to 7.89 mg • g–1 dw), β-carotene (0.16 mg • g–1 to 0.78 mg • g–1 dw). These results suggest that D. dentata is likely potential source material to explore the industrial utilization, especially functional food and biomedical ingredients, of Fuco and Chl a.