Solvent Optimization For Genistein Isolation Of “Rotten Tempe†By High Performance Liquid Chromatography Method
| Main Authors: | Riyanto, Cucun Alep, Soetjipto, Hartati |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Matematika dan Ilmu Pengetahuan Alam
, 2017
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| Online Access: |
https://journal.uii.ac.id/Eksakta/article/view/8311 https://journal.uii.ac.id/Eksakta/article/view/8311/7320 https://journal.uii.ac.id/Eksakta/article/downloadSuppFile/8311/384 |
Daftar Isi:
- Genistein is a soy isoflavone that has been known have anticancer properties. The aim of this research is to determine the right combination of solvents to extract isoflavones in rotten tempe and determine the genistein content of the extraction process by the High Performance Liquid Chromatography (HPLC) method. Isoflavone extraction process was done using a mixture of methanol and chloroform solutions. Isoflavone extracts obtained will be analysed using High Performance Liquid Chromatography (HPLC) method to determine the content of genistein. The optimum isoflavone extract was obtained from chloroform:methanol (10:1 v/v) solvent ratio. Where as the highest contents of genistein from the 4th day “rotten tempe†as 26.199 ± 25.146 (mg/g).