Uji potensi dan optimasi konsentrasi lignoselulosa beberapa limbah pertanian sebagai sumber karbon dalam produksi enzim b-glukosidase Phanerochaete chrysosporium / Wuri Damayanti
| Main Author: | Damayanti, Wuri |
|---|---|
| Format: | Thesis NonPeerReviewed |
| Terbitan: |
, 2014
|
| Subjects: | |
| Online Access: |
http://repository.um.ac.id/23786/ |
Daftar Isi:
- DamayantiW.PotentialTestandOptimizationConcentrationofLignocellulosicfromVariousAgriculturalWasteasCarbonSourceonEnzyme946-glucosidaseProductionfromPhanerochaetechrysosporium.UndergraduatedDepartmentofChemistryFacultyofMathematicsandNaturalScienceStateUniversityofMalang.Advisors(1)EviSusantiS.Si.M.Si.(2)Dr.SuhartiM.Si.KataKunci946-glukosidaselignocellulosicagriculturalwastecarbonsourcePhanerochaetechrysosporium12288122881228812288Enzyme946-glucosidaseistheenzymethatcatalyzeshydrolysisreactioncellobiosatoglucose.Enzyme946-glucosidaseproductionfromP.chrysosporiumisstronglyinfluencedbythetypeofcarbonsourceinthemediaproduction.Theaimedofthisresearchedwereexaminescientificinformationabout(1)themostpotentialcarbonsourceamonglignocellulosicagriculturalwastecornstoverbagassericehuskricestrawandsawdustin946-glucosidaseproductionfromP.chrysosporium(2)optimizationconcentrationofthemostpotentiallignocellulosicagriculturalwasteascarbonsourcein946-glucosidaseproductionfromP.chrysosporium12288122881228812288ThisresearcharelaboratorythatconductedinDepartmenofChemistryStateUniversityofMalangandMicrobiologyLaboratoryStateUniversityofBrawijayaMalang.Thephaseofthisresearchare(1)samplepreparation(lignocellulosic)ascarbonsourcearericestrawricehuskcornstoverbagasseandsawdust(2)rejuvenationpurecultureofP.chrysosporium(3)manufactureofP.chrysosporiumsporesuspension(4)946-glukosidaseproductionfromP.chrysosporiumusinglignocellulosicascarbonsource(5)determinationtheoptimumconcentrationofthemostpotentialcarbonsourcetoproduce946-glukosidaseenzymefromP.chrysosporium.Lignocellulosicconcentrationusedare003050710dan12%(b/v).Selectionthemostpotentialcarbonsourceanddeterminationtheoptimumconcentrationbasedonthelevelof946-glucosidaseactivity.The946-glucosidaseactivitywasdeterminedbyreductionoftheamountofsugarproducedafterthereaction.ReducingsugarcontentwasdeterminedbyusingtheDNSmethod.12288122881228812288Theresultofthisresearchshowedthatricestrawricehuskcornstoverbagasseandsawdusthavethepotentialascarbonsourceon946-glucosidaseproductionfromP.chrysosporium.Theuseofwastecornstoverbagassericestrawricehuskandsawdustataconcentrationof1%canproduceconsecutivethehighest946-glucosidaseactivity2169U/mL4853U/mL12132U/mL500U/mLand6691U/mL.Thericestrawisthemostpotentiallignocellulosicwasteascarbonsourceon946-glukosidaseproductionfromP.chrysosporium.Thericestrawcanproducethehighest946-glukosidaseactivityat1%asbigas12132U/mL.12288122881228812288