UJI SEROLOGI ISOLAT BACILLUS THURINGIENSIS DAN PATOGENISITASNYA TERHADAP JENTIK NYAMUK VEKTOR

Main Authors: P., Blondie Ch., U, Widyastuti, Widiarti, Widiarti, Sukarno, Sukarno, Subiantoro, Subiantoro
Other Authors: BADAN LITBANGKES KEMENKES
Format: application/pdf eJournal
Bahasa: ind
Terbitan: Badan Penelitian dan Pengembangan Kesehatan , 2012
Subjects:
Online Access: http://ejournal.litbang.depkes.go.id/index.php/BPK/article/view/293
Daftar Isi:
  • Serology test study of 20 Bacillus thuringiensis bacteria isolates and it's pathogenicity against vector mosquito larvae last instar III, on samples from various soil habitate was done in Salatiga municipal, Semarang regency, Purworejo regency and East Flores regency. The method used in this study (to determine Bacillus thuringiensis isolate serotype) was based on H-antigen (flagela) which is used as a movement organ of the bacteria. The twenty Bacillus thuringiensis isolates which were tested, can be grouped to 11 serotype (serotype H-3, 14, 43, 10, 8, 24, 11, 6, 23, 28, 13 and 11). H-14 serotype is the dominant serotype (3 isolate) followed by H-3, 43, 10 and 23 serotype are 2 isolate respectively and H-8, 24, 11, 6, 28 and 13 serotype are 1 isolate respectively. The pathogenicity test of 3 Bacillus thuringiensis isolate H-14 serotype against Aedes aegypti and Culex quinquefasciatus mosquito larvae, showed that 3 isolates (100%) has pathogenecity at 82.7% - 94.7% and 64.0% - 93.3% for 24 hours of exposure respectively. In 48 hours of exposure, using the same test, results showed that 3 isolates (100%) have a pathogenicity at 84.0% - 98.7% and 81.3% - 96.0% respectively.