Kontribusi peroksidasi lipid terhadap kerusakan sel hati tikus putih akibat keracunan Aflatoksin B1
| Main Author: | Yanwirasti, Yanwirasti |
|---|---|
| Format: | Article info application/pdf Journal |
| Bahasa: | eng |
| Terbitan: |
Jurnal Anatomi Indonesia
, 2012
|
| Online Access: |
https://journal.ugm.ac.id/jai/article/view/1123 https://journal.ugm.ac.id/jai/article/view/1123/943 |
Daftar Isi:
- The experiment used 96 white rats (Rattus norvegicus) with age around eight weeks old and weight 180-200grams, divided into four groups of 24 rats each, based on the dosages ofAflatoxin B1 given. Each group wasdivided further into three subgroups of eight rats based on the length of exposure time ofAflatoxin1.Four dosages ofAflatoxin1 were administered orallyeveryday into different groups, consisted of 0 μg, 10 μg,15 μg, and 20 μg, dissolved in 0.2ml propylene glycol. Three subgroups received the dosage for 12 weeks, 16weeks, and 20 weeks.At the end of the experiment, the rats were sacrificed,malondialdehyde were analyzedusing Uchiyama and Mihara method. Liver cell damages were examined using histological slices stained byhaematoxilin eosin. Data was analyzed using analysis of variance, and P<0.05 was considered to be significantlydifferent.Data analysis shows that : 1) there were significant differences between the effects of 12 weeks and 20weeks exposure, and between dosage of 10 μg and 20 μg on increasingmalondialdehyde of liver tissue andliver cell damage. This also showed that increasing exposure time and dosages of Aflatoxin1 increasing inmalondialdehyde of liver tissue and liver cell damage. 2) There were no significant differences between dosages10 μg and 15 μg, between 15 μg and 20 μg, between 12 weeks and 16 weeks, and between 16 and 20weeks exposures, on increasingmalondialdehyde of the liver tissue. 3) themoreAflatoxin1 was given themoreliver cells damage. It is concluded that aflatoxin1may cause liver cell damage as the result of liver peroxidationand many other factors as well.