Karakteristik dan komposisi semen kancil (Tragulus javanicus) yang dikoleksi dengan elektroejakulator
| Main Authors: | Prasetyaningtyas, Wahono Esthi, Setiadi, Mohamad Agus, Fahrudin, Mokhamad, Haron, Abdul Wahid, Agungpriyono, Srihadi |
|---|---|
| Format: | Article info application/pdf Journal |
| Bahasa: | eng |
| Terbitan: |
Jurnal Anatomi Indonesia
, 2012
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| Online Access: |
https://journal.ugm.ac.id/jai/article/view/1115 https://journal.ugm.ac.id/jai/article/view/1115/939 |
Daftar Isi:
- The ejaculates were taken by electroejaculation from four apparently healthy young adultmale lessermousedeer (Tragulus javanicus). The animals were anesthetized with a combination of xylazin and ketamine followedby ether per inhalation anesthesia. The semen was white, yellowish, or creamy in color. The semen had a pH of7.63±0.22. Themean values for volumewas 19.44±6.8 μl, spermconcentration was 47.44 ± 4.9 x 106 sperm/ml,percentage of sperm motility was 36.43±1.1 %, percentage of live sperm was 53.11±3.0 %, percentage ofabnormal spermatozoa was 21.03±1.05%and percentage of intact acrosome was 52.28±2.7%, respectively.These values were relatively lowwhen compared to other domestic ruminants and suggested to be relatedwithage and sexualmaturity of the animal.The seminal plasma contained 10.2–11.5mg/100ml fructose, 22.07–24.5mg/100ml citric acid, 65mg/100ml proteins, 22.07–24.5mg/100ml sorbitol, 91.1– 94.72mg/100ml sodium, 0.1mg/100ml potassium, 12.8mg/100ml calcium, 0.8mg/100mlmagnesiumand 10.72–11.2 chloride. By SDS PAGE,eleven proteins with different molecular weight were determined in the seminal plasma. Among them, theproteins with 64.77 kDa and 71.72 kDa were particularly prominent.