Pemberian alkohol peroral secara kronis menurunkan kepadatan sel granula cerebellumpada tikus putih (Rattus norvegicus) jantan dewasa
| Main Authors: | Halim, Hendry, Fakhrurrazy, Fakhrurrazy, Yuliastuti, Yuliastuti, Sari, Dwi Cahyani Ratna, Susilowati, Rina |
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| Format: | Article info application/pdf Journal |
| Bahasa: | eng |
| Terbitan: |
Jurnal Anatomi Indonesia
, 2012
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| Online Access: |
https://journal.ugm.ac.id/jai/article/view/1113 https://journal.ugm.ac.id/jai/article/view/1113/937 |
Daftar Isi:
- Background: Chronic exposure of alcohol inducesmassive degeneration of cells in the rat cerebellar cortex.Objective: This study was performed to know the effect of chronic alcohol exposure on granule cells densityin the cerebellar cortex.Materials and Methods: Twenty 10-12 weeks old-male-Wistar’ rats were dividedrandomly into four groups.Group I, the control group,was given aquadest 2mL/day.Each rats of group II, III, andIV was given alcohol 3%, 12%, and 20% 2mL/day, respectively.After 30 days, rats were deeply anesthetizedwith chloralhydrate 3,5 % before transcardial perfusion with fixative was conducted. Brains were then removed,processed for paraffin embedding and toluidine blue staining. The average density of granule cellswascounted in six random10.000 μm2 regions in the granule cell layer of anterior, posterior and flocculonodular lobesofmidline section of cerebellum.Results:The number of cerebellar granule cell per 10.000mm2were 354,1±14,1;331,9±20,9 ; 297,7±10,2; 279,5±9,3 for group I, II, III, and IV respectively (F = 27,1; df = 3; p < 0,05). Conclusion:Chronic alcohol exposure decreased the density of cerebellar granule cells in adultmale rat.