Comparative Determination of Total Phenolic Content and Flavonoid Content of Hanopol (Poikilospermum suaveolens) Using Water and Aqueous Ethanol as Extracting Solvents
| Main Authors: | Francisco, Glemcie Jane V., Pajarito, Dexter F. |
|---|---|
| Format: | Article info application/pdf Proceeding |
| Bahasa: | eng |
| Terbitan: |
LPPM Universitas Advent Indonesia
, 2018
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| Online Access: |
https://jurnal.unai.edu/index.php/isc/article/view/1214 https://jurnal.unai.edu/index.php/isc/article/view/1214/743 |
Daftar Isi:
- Phenolic compounds which are ubiquitous in plants have been shown to exhibit antioxidant activity. Consequently, these compounds may reduce the risk of degenerative diseases including certain types of cancer and heart diseases. Since the phenolic contents of Hanopol (Poikilospermum suaveolens) have not been widelyexplored, this study was conducted to compare to the total phenolic content (TPC)and total flavonoid content (TFC) of water and aqueous ethanol crude extracts of thedried leaves and vines of Hanopol using Folin-Ciocalteau and Aluminum chloridecolorimetric assays, respectively. The leaves and vines extracted with aqueousethanol as solvent showed TPC values of 485.4 and 148.0 mg gallic acid equivalent,respectively, higher than extracted with water only with 63.9 mg and 56.2 mg gallicacid equivalent, respectively. Total flavonoid content in the leaves and vinesextracted with aqueous ethanol were 152.3 and 24.9 mg catechin equivalent,respectively while leaves and vines extracted with water only showed significantlylower TFC (p <0.001, Tukey’s post-hoc test). Results of this study showed that theenhanced polarity of aqueous ethanol exhibited better extracting capacity than wateronly towards phenolic compounds. The use of liquid chromatographic technique toseparate individual phenolics compounds and identification of molecular structureusing mass spectrometry are warranted for future studies.