REGENERASI IN VITRO TANAMAN TEBU (Saccharum officinarum L.): INDUKSI DAN PROLIFERASI KALUS, SERTA INDUKSI
| Main Authors: | Ronald Bunga Mayang, Dwi Hapsoro, . Yusnita2 |
|---|---|
| Format: | Article application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
JURNAL AGROTROPIKA
, 2013
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| Subjects: | |
| Online Access: |
http://journal.unila.ac.id/index.php/agrotropika/article/view/679 |
Daftar Isi:
- IN VITRO PLANT REGENERATION OF SUGARCANE (Saccharum officinarum L.): CALLUSINDUCTION, PROLIFERATION, AND SHOOT INDUCTION. This research is a series of activities thatstudies the embryogenesis for somatic clonal reproduction plant sugarcane multiplication in vitro which includesthe callus induction of explant pieces rolls up the leaves, the proliferation of embriogenic callus, andshoots induction of embriogenic callus. Those of three researchs were conducted in a series. This experimentwere carried out in The Plant Science Laboratory, the Faculty of Agriculture, the University of Lampung,Indonesia, from Oktober 2010 until April 2011. All experiments were conducted in a completely randomized design. Treatments of Experiment I were 2,4-D concentrations (0, 1, 2, and 3 mg/l) with and without 250 mg/l of casein hydrolysate, while those of Experiment II were combinations of those two chemicals,2,4-D concentrations (1, 2, and 3 mg/l) with and without 250 mg/l of casein hydrolysate. Treatments of Experiment III were BA concentrations (1 mg/l and 2.5 mg/l). Results of the experiments showed that the best callus induction was achieved using MS media containing 3 mg/l 2,4-D, and callus induction was not significantly affected by casein hidrolysates. Concentrations of 2,4-D did not significantly influence embryogenic callus proliferation. The best shoot proliferation was attained in MS media containing 2.5 mg/l.