Toxicity testings of isolated Bacillus sp. against mosquito larvae in the laboratory
| Main Author: | Sugeng Juwono Mardihusodo, Sugeng Juwono Mardihusodo |
|---|---|
| Format: | Article info application/pdf |
| Bahasa: | ind |
| Terbitan: |
Journal of the Medical Sciences (Berkala ilmu Kedokteran)
, 2015
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| Online Access: |
https://journal.ugm.ac.id/bik/article/view/4489 https://journal.ugm.ac.id/bik/article/view/4489/3758 |
Daftar Isi:
- Studies on the spectrum of insecticidal activities of any entomopathogens indigenously obtained from any mosquito larval habitats should be accomplished to realize its recpective degree of potency and specificity as a biological control agent.These studies aimed at testing of comparative toxicides of four bacilli isolates: B. sphaericus I (23A), B. sphaericus11 (51C), B. pumilus (25C) and B. cereus (142A) against Cul= quingu.efasciatus, Aedes aegypti and Anopheles aconitus larvae in laboratory conditions.Pathogenicity and toxicity testings were undertaken after the four bacilli isolates and their controls (B. thuringiensis H-14 and B. sphaericus 1593) being cultured in slant media or produced through fermentations with a defined medium (Prof Sam Singer) as final whole cultures (FWCs) and primary powders. Titrations of all primary powders produced were carried out against standard powders of B. t. H-14 (IPS 82) and B. sphaericus 1593 (RB 80).The results of the testings showed that:1. the four bacilli isolates were respectively pathogenic for Cx. quinquefasciatus, Ae. aegypti and An. aconitus larvae;2. the degree of sensitivities of the three species of mosquito larvae against the four bacilli isolates from higher to lower levels were Cx. quinquefasciatus, Ae. aegypti and An. am-nib's;3. toxicides of the four bacilli isolates were still much lower than that of either B. t. H-14 or B. sphaericus 1593, and4. results of the attempts of primary powder production with a Sam Singer's medium for the four isolates, B. I. H-14 and B. sphaericus 1593 were considered satisfactory.Key Words: entomopathogens - bacilli isolates - B. thuringiensisH-14 - B. sphaericus 1593 - biological