Analysis of Melamine Using Electronic Nose, Gas Chromatography-Time of Flight - Mass Spectrometry and High Performance Liquid Chromatography
| Main Authors: | Rohman., Abdul, Che Man, Yaakob B., Hafidz, R. M. |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Farmasi, Universitas Gadjah Mada
, 2013
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| Online Access: |
http://journal.ugm.ac.id/jfps/article/view/1843 http://journal.ugm.ac.id/jfps/article/view/1843/1650 |
Daftar Isi:
- An electronic nose (E-Nose) has been used as rapid analytical technique for melamine detection in food samples. The parameter used is melamine retention index (MRI) in E-Nose chromatogram. The value of MRI was also confirmed using two dimensional gas chromatography-time of flight-mass spectrometry (GCxGC-TOF-MS). The value of MRI using E-Nose and GCxGC-TOF-MS was nearly close i.e 1486 and 1540 respectively. HPLC has been used for quantitative analysis of melamine in some milk products suspected to contain melamine using column Nova-Pak® C18;(150 x 3.9 mm i.d; 4 μm) at 30°C. The mobile phase was 5 mmol L−1 NaH2PO4 delivered isocratically at flow rate of 1 mLmin-1 . Melamine was linear at concentration range of 0.0625 – 50.0 μg/ml with coefficient of determination (R2 ) of 0.999.