Ethanol Extract of Stylissa carteri Induces Cell Death in Parental and Paclitaxel-Resistant Cervical Cancer Cells
| Main Authors: | Hardani, Isna Nisrina, Damara, Fachreza Aryo, Nugrahani, Annisa Dewi, Bashari, Muhammad Hasan |
|---|---|
| Other Authors: | Beginner Subhan, Bogor Agricultural University, Agus Erdina, Bogor Agricultural University, Harold Atmaja, Bogor Agricultural University, Pratiwi, Dr. Hasan Sadikin Hospital, Tenny Putri, Laboratory of Cell Culture and Cytogenetic |
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
International Journal of Integrated Health Sciences
, 2018
|
| Subjects: | |
| Online Access: |
http://journal.fk.unpad.ac.id/index.php/ijihs/article/view/1378 http://journal.fk.unpad.ac.id/index.php/ijihs/article/view/1378/1209 http://journal.fk.unpad.ac.id/index.php/ijihs/article/downloadSuppFile/1378/877 http://journal.fk.unpad.ac.id/index.php/ijihs/article/downloadSuppFile/1378/878 http://journal.fk.unpad.ac.id/index.php/ijihs/article/downloadSuppFile/1378/879 http://journal.fk.unpad.ac.id/index.php/ijihs/article/downloadSuppFile/1378/3019 |
Daftar Isi:
- Objective: To evaluate the cytotoxic effects of ethanol extract from marine sponge Stylissa carteri in both parental and paclitaxel-resistant HeLa cervical cancer cells. Methods: This was an experimental in-vitro study subjected ethanol extract from Stylissa carteri obtained from Pramuka Island, Kepulauan Seribu National Park, Jakarta. Both parental and paclitaxel-resistant HeLa cells were treated with ethanol extract followed by microscopic observation and MTT assay. The IC50 and drug curves were analyzed using four-parametric logistic regression by SigmaPlot for Windows version 12.0 from Systat Software Inc., USA.Results: Ethanol extract from Stylissa carteri triggered cell death-associated morphological changes in parental Hela cells. It demonstrated cytotoxic activities with an IC50 value of 1 ppm. Importantly, this extract also triggered cell death in paclitaxel-resistant HeLa cells. The IC50 of ethanol extract of Stylissa carteri was 4 ppm in paclitaxel-resistant HeLa cells.Conclusions: There is a potential cytotoxic activity of Stylissa carteri that is not only confirmed in parental HeLa cells but also in paclitaxel-resistant HeLa cells.Keywords: Cervical cancer, paclitaxel resistance, HeLa cells, cytotoxicity assay, Stylissa carteri DOI: 10.15850/ijihs.v6n2.1378