DEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR DETERMINATION AND QUANTIFICATION OF RUBRAXANTHONE IN STEM BARK EXTRACT OF MANGOSTEEN
| Main Authors: | Susanti, Meri; Faculty of Pharmacy, Andalas University, Kampus Limau Manis, Padang -25163 PO. Box 143, Indonesia, Lena, Dwisesaria Irma; Faculty of Pharmacy, Andalas University, Kampus Limau Manis, Padang -25163 PO. Box 143, Indonesia, Dachriyanus, Dachriyanus; Faculty of Pharmacy, Andalas University, Kampus Limau Manis, Padang -25163 PO. Box 143, Indonesia |
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| Format: | Article info eJournal |
| Bahasa: | eng |
| Terbitan: |
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
, 2014
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| Online Access: |
http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/273 http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/273/153 |
Daftar Isi:
- Rubraxanthone is one of antibacterial and antioxidant compound which is isolated from stem bark of mangosteen (Garcinia mangostana Linn.). The precise method for analysis of plant constituents is normally a reverse-phase high performance liquid chromatographic (RP-HPLC). Development and validation of a RP-HPLC method for chromatographic separation was carried out on a reversed-phase column Shimadzu ® Shimp-pack VP – ODS (4.6x250mm) using an isocratic mobile phase of 0.4% formic acid - acetonitrile at a flow rate 1mL min-1, and detection was with a UV detector. The linearity of the proposed method was found in the range of 2.5-25μg/mL-1 with regression coefficient 0.999. Intraday precision studies showed the relative standard deviation ≤1.58% and inter-day ≤3.20%. Accuracy of the method was determined by a recovery study conducted at 3 different levels, and the average recovery was 102.18%. The Limit of Detection Limit of Quantitation (LOQ) and were 0.47 and 1.56μg/mL-1.The contents of rubraxanthon in the crude was 0.23±0.07%w/w.