Curcumin Analogs Induce Apoptosis and G2/M Arrest In 4T1 Murine Triple-Negative Breast Cancer Cells
| Main Authors: | Murwanti, Retno; Universitas Gadjah Mada, Rahmadani, Azmi; Universitas Gadjah Mada, Ritmaleni, Ritmaleni; Universitas Gadjah Mada, Hermawan, Adam; Universitas Gadjah Mada, Sudarmanto, Bambang Sulistiyo Ari; Universitas Gadjah Mada |
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| Other Authors: | Ministry of Research, Technology and Higher Education of the Republic of Indonesia |
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
, 2020
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| Subjects: | |
| Online Access: |
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1652 https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1652/938 |
Daftar Isi:
- Chemotherapy is the first-line treatment for triple-negative breast cancer (TNBC), yet toxicity and resistance effects have been the current problems. Curcumin,a natural compound, has been reported to exert anti-proliferative effects on various cancer cells, including breast carcinoma cells. However, the β-diketone moiety influences the stability of curcumin. Curcumin analogs, pentagamavunon-0 (PGV-0), and pentagamavunon-1 (PGV-1) were synthesized to improve the stability and activity of curcumin by modified the β-diketone moiety into mono-ketone pentanone. In this study, we evaluated the cytotoxicity, inhibition of cell cycle progression, and induction of apoptosis of curcumin and its analogs (PGV-0 and PGV-1) in murine triple-negative breast cancer 4T1 cell line. The cytotoxic evaluation was done by MTT assay, while apoptosis induction and cell cycle evaluation was performed by annexin V staining and detected by flow cytometry. Curcumin and its analogs, PGV-0, and PGV-1, significantly inhibit the viability of 4T1 breast cancer cells with an IC50 value of 34.34μg/mL, 13.76μg/mL and 38.21μg/mL, respectively. Apoptosis analysis with a dose of 10μg/mL and 15μg/mL in 4T1 breast cancer cells showed that curcumin and its analogs effectively induce apoptotic in a dose-dependent manner. In cell cycle analysis using a dose of 15μg/mL, curcumin inhibited the cell cycle progression in the S phase, whereas PGV-0 and PGV-1 inhibited the cell cycle in the G2/M phase. It could be concluded that curcumin analogs, PGV-0 and PGV-1, have higher potential to be developed as anti-cancer agents by inducing cell cycle arrest and apoptosis in triple-negative breast cancer.